JCRB9030 P3X63-Ag8.653

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9030 Cell Name   P3X63-Ag8.653
Profile   myeloma, non-secreting, 8-azaguanine resistant, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   non-secreting myeloma cells, partner of hybridoma
Life Span    Crisis PDL   
Morphology    Character   non-secreting myeloma cells, partner of hybridoma
Classify    Established by   Kearney, J. et al
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL1580. Year   
Medium   RPMI1640 medium with 20% fetal calf serum Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtained.
Cell Number on Passage   3-5 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4032
Reference
Pubmed id:113458A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
Kearney JF,Radbruch A,Liesegang B,Rajewsky K
J Immunol. 1979 Oct;123(4):1548-50
Pubmed id:74805Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse.
Lemke H,Hammerling GJ,Hohmann C,Rajewsky K
Nature. 1978 Jan 19;271(5642):249-51
Pubmed id:268647Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.
Koprowski H,Gerhard W,Croce CM
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8
Pubmed id:305123Fusion of T and B cells.
Köhler G,Pearson T,Milstein C
Somatic Cell Genet. 1977 May;3(3):303-12
Pubmed id:1172191Continuous cultures of fused cells secreting antibody of predefined specificity.
Köhler G,Milstein C
Nature. 1975 Aug 7;256(5517):495-7
Pubmed id:5439579Mouse myelomas and lymphomas in culture.
Horibata K,Harris AW
Exp Cell Res. 1970 Apr;60(1):61-77

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9030 Cell Name   P3X63-Ag8.653
LOT No.   041299 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (FBS lot; BioWhittaker 6M0121)　The vials are labeled as P3X63-Ag8. Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   D.T. = ca 17 hrs Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD. AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Labeled as P3X63-AG8
Cell No.   JCRB9030 Cell Name   P3X63-Ag8.653
LOT No.   020194 Lot Specification   distribution
Medium   RPMI1640 medium with 20% heat inactivated fetal bovine serum (JRS DO10400) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   73.4 Antibiotics Used   free
Passage Number   *P6 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LD, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9030 Cell Name   P3X63-Ag8.653
LOT No.   061990 Lot Specification   distribution
Medium   RPMI1640 + FCS 20% Temperature   37
Cell Density at Seeding   3-5 x 10^5/ml Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtained.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9030 Cell Name   P3X63-Ag8.653
LOT No.   063086 Lot Specification   distribution
Medium   RPMI1640 + FCS 20% Temperature   37
Cell Density at Seeding   3-5 x 10^5/ml Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtained.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   71% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Vials are labeled as [P3X63]
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