JCRB9029 MDCK (NBL-2)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9029 Cell Name   MDCK (NBL-2)
Profile   established from normal kidney, susceptible for many viral species Other Name   
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney, epithelial like morphology
Life Span    Crisis PDL   
Morphology    Character   kidney, epithelial like morphology
Classify    Established by   Madin,S.H.&Darby,N.B
Registered by    Regulation for Distribution   
Comment   Prepared from CCL 34. Year   
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% Trypsin.
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0422
Reference
Pubmed id:15234806Comparative study of influenza virus replication in Vero and MDCK cell lines.
Youil R,Su Q,Toner TJ,Szymkowiak C,Kwan WS,Rubin B,Petrukhin L,Kiseleva I,Shaw AR,DiStefano D
J Virol Methods. 2004 Sep 1;120(1):23-31
Pubmed id:567227Polarized monolayers formed by epithelial cells on a permeable and translucent support.
Cereijido M,Robbins ES,Dolan WJ,Rotunno CA,Sabatini DD
J Cell Biol. 1978 Jun;77(3):853-80
Pubmed id:828141The origin and characteristics of a pig kidney cell strain, LLC-PK.
Hull RN,Cherry WR,Weaver GW
In Vitro. 1976 Oct;12(10):670-7
Pubmed id:4248968A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium.
Leighton J,Estes LW,Mansukhani S,Brada Z
Cancer. 1970 Nov;26(5):1022-8
Pubmed id:5918973Characterization of an established line of canine kidney cells (MDCK).
Gaush CR,Hard WL,Smith TF
Proc Soc Exp Biol Med. 1966 Jul;122(3):931-5

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9029 Cell Name   
LOT No.   08302007 Lot Specification   distribution
Medium   Eagle's minimam essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). Cell name described on vial label is mistake. Correctly, MDCK (NBL-2). Temperature   37 C
Cell Density at Seeding   4 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA (at 37 C for 5-15 min). Very tightly attach to substratum.
Doubling Time   20 - 25 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P59 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as dog by NP, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9029 Cell Name   
LOT No.   06082000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   3-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   20-24 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P58 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as dog by NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO and 20% FCS CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9029 Cell Name   MDCK (NBL-2)
LOT No.   080896 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9029 Cell Name   MDCK (NBL-2)
LOT No.   100594 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (JR Scientific CO19407) Temperature   37 C
Cell Density at Seeding   2.5x10^4 cells/sq.cm. Methods for Passages   Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA at 37C for 20-35min.
Doubling Time   13.9 hrs Cell Number in Vial (cells/1ml)   3.41x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P58 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LD, NP tested (dog)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9029 Cell Name   MDCK (NBL-2)
LOT No.   071492 Lot Specification   distribution
Medium   E.MEM (lot.62992) + FCS 10% (lot.ICN Flow101083) Temperature   37 C
Cell Density at Seeding   1x10^6 Methods for Passages   Wash cells once with PBS and treated with 0.02% EDTA + 0.25% trypsin at 37 C for 15min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.92x10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   P57 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9029 Cell Name   MDCK(NBL-2)
LOT No.   021389 Lot Specification   distribution
Medium   MEM + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9029 Cell Name   MDCK (NBL-2)
LOT No.   10232013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   5.8 - 6.1 x 10^4 cells/mL Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% Trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6 cells/1mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P59 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)   97.4 Additional information   
Cell No.   JCRB9029 Cell Name   MDCK (NBL-2)
LOT No.   08052019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA (at 37 C for 5-15 min). This cell line will very tightly attach to substratum, and .
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98.8 Additional information   
Cell No.   JCRB9029 Cell Name   MDCK (NBL-2)
LOT No.   10272021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P58 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   
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