JCRB9026 L1210
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9026 | Cell Name | L1210 |
|---|---|---|---|
| Profile | leukemia, lymphocytic, (DBA/2 mouse) | Other Name | |
| Animal | mouse | Strain | DBA/2 |
| Genus | Mus | Species | musculus |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | lymphocytic leukemic cells | |
| Life Span | Crisis PDL | ||
| Morphology | Character | lymphocytic leukemic cells | |
| Classify | Established by | Law,E. et al. | |
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from CCL219. | Year | 1986 |
| Medium | Fisher's medium with 10% horse serum. | Methods for Passages | Dilution. A maximum of 1-2 x 10^6/ml is obtainable. |
| Cell Number on Passage | 4-6 x 10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | spleen lymph node |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:6054389 | Strengthening dietetic management practices through idea-sharing. Fallon BJ,Watson HW J Am Diet Assoc. 1967 Nov;51(5):451-2 |
| Pubmed id:13823721 | Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines. FOLEY GE,DROLET BP,McCARTHY RE,GOULET KA,DOKOS JM,FILLER DA Cancer Res. 1960 Jul;20():930-9 |
| Pubmed id:18143260 | Observations on the effect of a folic-acid antagonist on transplantable lymphoid leukemias in mice. LAW LW,DUNN TB J Natl Cancer Inst. 1949 Aug;10(1):179-92 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9026 | Cell Name | |
|---|---|---|---|
| LOT No. | 09012006 | Lot Specification | distribution |
| Medium | Fischer's medium with 10% horse serum | Temperature | 37 C |
| Cell Density at Seeding | 1 - 2 x 10^5 cells/ml | Methods for Passages | Dilution |
| Doubling Time | approx. 34 hrs | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 |
| Viability at cell freezing (%) | 97.4 | Antibiotics Used | free |
| Passage Number | P384 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% HS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9026 | Cell Name | L1210 |
|---|---|---|---|
| LOT No. | 083093 | Lot Specification | distribution |
| Medium | Fischer's medium with 10% horse serum (ICN/Flow 0130293) | Temperature | 37 C |
| Cell Density at Seeding | 1-3x10^5 cells/ml | Methods for Passages | Harvest cells and dilute to 5-10x10^4/ml. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.2x10^6 |
| Viability at cell freezing (%) | 82.5 | Antibiotics Used | freee |
| Passage Number | P383 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | G6PD, PepB |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9026 | Cell Name | L1210 |
|---|---|---|---|
| LOT No. | 020189 | Lot Specification | distribution |
| Medium | Fisher's medium + horse serum 10% | Temperature | 37 |
| Cell Density at Seeding | 4-6 x 10^4/ml | Methods for Passages | Dilution. A maximum of 1-2 x 10^6/ml is obtainable. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.0x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P383 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |