JCRB9022 CPAE

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9022 Cell Name   CPAE
Profile   pulmonary artery normal endothelial cell Other Name   CPAE
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex   F Age   
Identity    Tissue for Primary Cancer   vascular system
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   -24
Morphology   epithelial-like Character   pulmonary artery endothelium
Classify   normal Established by   Del Vecchio,P.
Registered by   Mizusawa,H. Regulation for Distribution   Comments: Collagen coated culture dish is required for this cell line.
Comment   Prepared from CCL209. Year   1985
Medium   Eagle's minimal essential medium with 20% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   pulmonary artery
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2877
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9022 Cell Name   
LOT No.   12172003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 20% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio = 1:3.
Doubling Time   D.T. = ca. 1.8 days Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   P23 PDL   11 at bank
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as bovine by NP, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   052395 Lot Specification   distribution
Medium   EMEM with 20% heat-inactivated FBS,100ug/ml heparin(novo-nordiks) & HUVE growth factor (Morinaga,Co) Temperature   37 C
Cell Density at Seeding   1.86x10^4 cells/sq.cm. Methods for Passages   Rinse with PBS(-) and treated with 0.25% trypsin-0.02% EDTA at 37 C for 5-20min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.37x10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   P21 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   100992 Lot Specification   distribution
Medium   Eagle's MEM with 20% FBS(Hyclone),100ug/ml heparin& 100-150ug/ml endotherial cell growth supplement. Temperature   37 C
Cell Density at Seeding   9.0x10^4 cells/ml Methods for Passages   Treat cells in dishes by 0.25% trypsin in 0.02% EDEDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   050891 Lot Specification   distribution
Medium   MEM + FCS 20% Temperature   37 C
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9022 Cell Name   CPAE
LOT No.   020888 Lot Specification   distribution
Medium   MEM + FCS 20% Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P18 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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