JCRB9020 BHK-21(C-13)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9020 Cell Name   BHK-21(C-13)
Profile   kidney, subclone of BHK-21 Other Name   BHK
Animal   hamster, Syrian Strain   
Genus   Mesocricetus Species   auratus
Sex    Age   1-day-old
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   kidney
Classify    Established by   Macpherson & Stoker
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepare from CCL10. Difco tryptose phosphate broth 29.5 g/l. Year   1985
Medium   Eagle's minimal essential medium with 10% calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 to 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1915
Reference
Pubmed id:14468055Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.
MACPHERSON I,STOKER M
Virology. 1962 Feb;16():147-51

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9020 Cell Name   
LOT No.   04022010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids (0.1 mM each) and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   89.2 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Syrian hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   
LOT No.   10012004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   6-9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 30 hrs Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as hamster(Syrian) by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   100796 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   072092 Lot Specification   distribution
Medium   BME with 10% Triptose phosphate(Difco)+10% CS(C.C.L.lot.00021-07) Filtered through 0.20 u membrane. Temperature   37 C
Cell Density at Seeding   8.18x10^5 Methods for Passages   Monolayers are washed with PBS(-) & treated with 0.25%trypsin-0.02%EDTA. Centrifuge in growth med.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.12x10^6
Viability at cell freezing (%)   90.6 Antibiotics Used   free
Passage Number   P57 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   081386 Lot Specification   distribution
Medium   BME 80%, Difco tryptose phosphate broth 10%, CS10% Temperature   37
Cell Density at Seeding   1:2 to 1:10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   98% Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   06172020 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   5.0 x 10^6
Viability at cell freezing (%)   93.6 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - basal culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   83.1 Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   09272023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   
Cell Density at Seeding   1 - 3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   6.5 x 10^6
Viability at cell freezing (%)   77 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   73.8 Additional information   
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