JCRB9013 VERO

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9013 Cell Name   VERO
Profile   normal, contact inhibited Other Name   Vero
Animal   monkey, African green Strain   African green
Genus   Cercopithecus Species   aethiops
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span    Crisis PDL   
Morphology    Character   kidney
Classify   CCL 81 Established by   Yasumura & Kawakita
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL 81. Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids and 5% fetal calf serum. Methods for Passages   Cells treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0059
Reference
Pubmed id:15938255A plaque neutralization method for arboviruses.
Earley E,Peralta PH,Johnson KM
Proc Soc Exp Biol Med. 1967 Jul;125(3):741-7
Pubmed id:4961494Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero).
Liebhaber H,Riordan JT,Horstmann DM
Proc Soc Exp Biol Med. 1967 Jun;125(2):636-43
Pubmed id:5591575Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures.
Rhim JS,Simizu B,Wiebenga NH
Arch Gesamte Virusforsch. 1967;21(2):243-52
Pubmed id:5833688Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay.
Sasaki K,Makino S,Kasahara S
Kitasato Arch Exp Med. 1964 Dec;37(1):27-42

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9013 Cell Name   
LOT No.   11292002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 5% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   < 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P131 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as African green monkey by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture meidum with 10% DMSO and 20% FBS CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9013 Cell Name   
LOT No.   041999 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   3-4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 30 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P130 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as African green monkey by NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9013 Cell Name   VERO
LOT No.   030592 Lot Specification   distribution
Medium   E.MEM with NEAA (lot.021292) + FBS 5% (J.R.Scientific lot.CO19407, Cell Cult.Lab. lot.CO00939-01) Temperature   37 C
Cell Density at Seeding   7.3x10^5 Methods for Passages   0.02% EDTA + 0.25% trypsin
Doubling Time   12 hr Cell Number in Vial (cells/1ml)   4.1x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P129 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9013 Cell Name   VERO
LOT No.   07082019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 5% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.8-1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P130 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   91.7 Additional information   
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