JCRB9007 VERO 76
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9007 | Cell Name | VERO 76 |
|---|---|---|---|
| Profile | normal, contact inhibited, lower saturation density than the original Vero | Other Name | |
| Animal | monkey, African green | Strain | African green |
| Genus | Cercopithecus | Species | aethiops |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | kidney | |
| Life Span | Crisis PDL | ||
| Morphology | Character | kidney | |
| Classify | Established by | ||
| Registered by | Mizusawa,H. | Regulation for Distribution | |
| Comment | Prepared from CRL 1587. | Year | 1985 |
| Medium | Dulbecco's modified Eagle's medium or Eagle's minimal essential medium with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin |
| Cell Number on Passage | split ratio=1/8 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2725683 | Mycoplasma infection of cultured cells. Hay RJ,Macy ML,Chen TR Nature. 1989 Jun 8;339(6224):487-8 |
| Pubmed id:4290661 | SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Girardi AJ,Weinstein D,Moorhead PS Ann Med Exp Biol Fenn. 1966;44(2):242-54 |
| Pubmed id:5833688 | Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay. Sasaki K,Makino S,Kasahara S Kitasato Arch Exp Med. 1964 Dec;37(1):27-42 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9007 | Cell Name | VERO 76 |
|---|---|---|---|
| LOT No. | 041387 | Lot Specification | distribution |
| Medium | DMEM or MEM + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 1/8 | Methods for Passages | 0.02% EDTA and 0.25% trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.7x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P32 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | AST,G6PD,LD,MD,MPI,NP,PEPB |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9007 | Cell Name | VERO 76 |
|---|---|---|---|
| LOT No. | 09072015 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). | Temperature | 37 C |
| Cell Density at Seeding | approx. 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | 25 - 44 hrs | Cell Number in Vial (cells/1ml) | 5.6 x 10^6 |
| Viability at cell freezing (%) | 90.2 | Antibiotics Used | free |
| Passage Number | P33 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90.2 | Additional information |