JCRB0831 HO-1-N-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0831 Cell Name   HO-1-N-1
Profile   squamous carcinoma, buccal mucosa Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   oral
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   squamous cell carcinoma
Classify    Established by   
Registered by    Regulation for Distribution   
Comment    Year   
Medium   Dulbecco's modified Eagle's medium - F12 mdeium (1:1 mix) with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.1% trypsin and 0.02% EDTA and dilute in fresh medium.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   buccal mucosa
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1284
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 924111
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0831 Cell Name   
LOT No.   04092001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium : Ham's F12 medium = 1:1 with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   Hardly detach. Wash 3 times with PBS(-) and treat cells with 0.25% trypsin and 0.02% EDTA in PBS(-) for 5-10 min at 37 C.
Doubling Time    Cell Number in Vial (cells/1ml)   7.8 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0831 Cell Name   
LOT No.   02292001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium : Ham's F12 medium = 1:1 with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   3-9 x 10^4 cells/ml Methods for Passages   Hardly detach. Wash 3 times with PBS(-) and treat cells with 0.25% trypsin and 0.02% EDTA in PBS(-) for 5-10 min at 37C.
Doubling Time    Cell Number in Vial (cells/1ml)   6.0 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P39 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0831 Cell Name   
LOT No.   090495 Lot Specification   distribution
Medium   Dulbecco's MEM : Ham's F12 = 1:1 mixture with 10% fetal bovine serum. Temperature   37
Cell Density at Seeding    Methods for Passages   monolayer culture
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0831 Cell Name   HO-1-N-1
LOT No.   08212015 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium - Ham's F12 mdeium (DMEM/F12) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   6.9 - 8.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9
D13S317:10,11
D7S820:10,13
D16S539:9,11
VWA:16,18
TH01:9
AM:X,Y
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   97.9 Additional information   
Cell No.   JCRB0831 Cell Name   HO-1-N-1
LOT No.   10232019 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.8 - 5.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   P38 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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