-Detailed Information [JCRB0826]-

JCRB0826 8505C

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0826	Cell Name	8505C
Profile	thyroid carcinoma, undifferentiated	Other Name	zero-go-see
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	78
Identity	available	Tissue for Primary Cancer	thyroid
Case history	undifferentiated thyroid carcinoma	Metastasis	No
Tissue Metastasized		Genetics	C:G->G:C transversion at codon 248 of p53 gene
Life Span	infinite	Crisis PDL
Morphology	fibroblast-like	Character
Classify	tumor	Established by	Nakamura,N.
Registered by	Akiyama,M.	Regulation for Distribution
Comment		Year	1988
Medium	Eagle's minimal essential medium with 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Cell Number on Passage	1.5x10^5 cells/sq.cm.	Race
CO2 Conc.	5 %	Tissue Sampling	thyroid
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1054
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	924102

Reference
Pubmed id:21566963	Establishment of 2 human thyroid-carcinoma cell-lines (8305c, 8505c) bearing p53 gene-mutations. Ito T,Seyama T,Hayashi Y,Hayashi T,Dohi K,Mizuno T,Iwamoto K,Tsuyama N,Nakamura N,Akiyama M Int J Oncol. 1994 Mar;4(3):583-6
Pubmed id:1737400	Unique association of p53 mutations with undifferentiated but not with differentiated carcinomas of the thyroid gland. Ito T,Seyama T,Mizuno T,Tsuyama N,Hayashi T,Hayashi Y,Dohi K,Nakamura N,Akiyama M Cancer Res. 1992 Mar 1;52(5):1369-71

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

03242008
11252003
081595
10192023

Cell No.	JCRB0826	Cell Name	8505C
LOT No.	03242008	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(Intergen RB52305)	Temperature	37 C
Cell Density at Seeding	1.9x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.3x10^6
Viability at cell freezing (%)	98.4	Antibiotics Used	NT
Passage Number	p29	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:10,11 D13S317:13 D7S820:10 D16S539:12 VWA:17,19 TH01:6,9 AM:X TPOX:11 CSF1PO:12,13
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0826	Cell Name	8505C
LOT No.	11252003	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	6-8 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T. = ca. 1.5 days	Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	95.7	Antibiotics Used	free
Passage Number	P27	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	98%	Additional information

Cell No.	JCRB0826	Cell Name
LOT No.	081595	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal calf serum	Temperature	37 C
Cell Density at Seeding		Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	>90	Antibiotics Used
Passage Number	P23	PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0826	Cell Name	8505C
LOT No.	10192023	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; NICHIREI Cat. # 174112)	Temperature	37 C
Cell Density at Seeding	6.0 - 9.1 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 37 hrs.	Cell Number in Vial (cells/1ml)	2.2 x 10^6
Viability at cell freezing (%)	100	Antibiotics Used	free
Passage Number	P29	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,11 D13S317:13 D7S820:10 D16S539:12 VWA:17,19 TH01:6,9 AM:X TPOX:11 CSF1PO:12,13
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	96	Additional information