JCRB0825 YMB-1-E

Cell information

Important Notice(s)

JCRB0823 YMB-1, JCRB0825 YMB-1-Eについてのご注意

JCRB0823 YMB-1, JCRB0825 YMB-1-E: the problem of origin

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0825 Cell Name   YMB-1-E
Profile   breast cancer, exhibit the same DNA profile as other breast carcinoma cell line ZR-75-1. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   55
Identity   available Tissue for Primary Cancer   mammary
Case history    Metastasis   
Tissue Metastasized    Genetics   breast cancer
Life Span    Crisis PDL   
Morphology    Character   breast cancer
Classify    Established by   Yamane,M.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   breast
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2815
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1303911
Reference
Pubmed id:6534930Establishment and characterization of new cell line (YMB-1) derived from human breast carcinoma.
Yamane M,Nishiki M,Kataoka T,Kishi N,Amano K,Nakagawa K,Okumichi T,Naito M,Ito A,Ezaki H
Hiroshima J Med Sci. 1984 Dec;33(4):715-20

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0825 Cell Name   
LOT No.   06142004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   0.6 - 1.2 x 10^5 cells/ml Methods for Passages   dilution (suspension culture)
Doubling Time   45 hrs Cell Number in Vial (cells/1ml)   8.2 x 10^5
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P69 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmes as human by NP, G6PD, MD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0825 Cell Name   
LOT No.   091195 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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