JCRB0824 8305C

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0824 Cell Name   8305C
Profile   Human undifferentiated thyroid carcinoma with C:G->T:A mutation at codon 273 of p53 gene. Other Name   5-C-O
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   67
Identity   available Tissue for Primary Cancer   thyroid
Case history   undifferentiated thyroid carcinoma Metastasis   No
Tissue Metastasized    Genetics   C:G->T:A mutation at codon 273 of p53 gene
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   thyroid anaplastic carcinoma, undifferentiated
Classify   tumor Established by   Hiraoka,T. et al.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   thyroid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1053
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 906795
Reference
Pubmed id:21566963Establishment of 2 human thyroid-carcinoma cell-lines (8305c, 8505c) bearing p53 gene-mutations.
Ito T,Seyama T,Hayashi Y,Hayashi T,Dohi K,Mizuno T,Iwamoto K,Tsuyama N,Nakamura N,Akiyama M
Int J Oncol. 1994 Mar;4(3):583-6
Pubmed id:1737400Unique association of p53 mutations with undifferentiated but not with differentiated carcinomas of the thyroid gland.
Ito T,Seyama T,Mizuno T,Tsuyama N,Hayashi T,Hayashi Y,Dohi K,Nakamura N,Akiyama M
Cancer Res. 1992 Mar 1;52(5):1369-71

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0824 Cell Name   
LOT No.   07252003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   3-6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 50 hrs Cell Number in Vial (cells/1ml)   9.0 x 10^5
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   P35 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0824 Cell Name   
LOT No.   090495 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0824 Cell Name   
LOT No.   091195 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0824 Cell Name   8305C
LOT No.   05302014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   3.7 - 8.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,13
D13S317:9
D7S820:8,10
D16S539:10,11
VWA:14,16
TH01:6,7
AM:X
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   86 Additional information   
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