JCRB0817 SBC-2

Cell information

Important Notice(s)

JCRB0816 SBC-1細胞とJCRB0817 SBC-2細胞の由来について

JCRB0816 SBC-1 and JCRB0817 SBC-2: the problem of origin

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0817 Cell Name   SBC-2
Profile   Stop providing for the issue of quality control. (Please contact us for more information) small cell carcinoma, intermediate, JCRB0816 SBC-1 and JCRB0817 SBC-2 exhibit the same DNA profile. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history    Metastasis   
Tissue Metastasized    Genetics   small cell carcinoma (intermediate)
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   small cell carcinoma (intermediate)
Classify    Established by   Hiraki,S.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 15% fetal calf serum Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_W531
Reference
Pubmed id:1330284In vitro measurement of chemosensitivity of human small cell lung and gastric cancer cell lines toward cell cycle phase-nonspecific agents under the clinically equivalent area under the curve.
Mitsuhashi Y,Inaba M,Sugiyama Y,Kobayashi T
Cancer. 1992 Nov 15;70(10):2540-6

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0817 Cell Name   
LOT No.   01212002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   2-7 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. (Easy to detach by pipetting without trypsin treatment)
Doubling Time   D.T. = ca 38 hrs Cell Number in Vial (cells/1ml)   5.0 x 10^6
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   P46 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0817 Cell Name   
LOT No.   081195 Lot Specification   distribution
Medium   RPMI1640 + 15% FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P53 (BM3) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0817 Cell Name   
LOT No.   091895 Lot Specification   distribution
Medium   RPMI1640 + 15% FCS Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptmycin
Passage Number   P39 (BM3) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
CLOSE