JCRB0736 Py-3Y1-S2
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0736 | Cell Name | Py-3Y1-S2 |
|---|---|---|---|
| Profile | 3Y1 derivative, mouse polyoma virus-transformed, (Fischer rat) | Other Name | |
| Animal | rat | Strain | Fischer |
| Genus | Rattus | Species | norvegicus |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | whole embryo, mouse polyoma virus-transformed 3Y1. | |
| Life Span | Crisis PDL | ||
| Morphology | Character | whole embryo, mouse polyoma virus-transformed 3Y1. | |
| Classify | new | Established by | Kimura,G. |
| Registered by | Regulation for Distribution | Citation of #966 is required. | |
| Comment | Year | 1988 | |
| Medium | Dulbecco (0.1% glucose) + 10% FCS | Methods for Passages | 0.05% trypsin + 5mM EDTA. Dilute 1/20. Prepare many frozen ampules when received. Use new stock afte |
| Cell Number on Passage | Less than 1x10^6 cells/50mm di | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | embryo/fetus whole |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2550745 | Frequency of cell transformation by the small DNA tumor viruses: infection of proliferating cells and quiescent cells. Matsuzaki A,Okuda A,Tamura H,Ohtsu M,Kimura G Microbiol Immunol. 1989;33(8):657-67 |
| Pubmed id:2844831 | Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents. Tanaka H,Zaitsu H,Onodera K,Kimura G J Cell Physiol. 1988 Sep;136(3):421-30 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0736 | Cell Name | |
|---|---|---|---|
| LOT No. | 08272004 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 3 x 10^4 cells/ml | Methods for Passages | 0.05% tryspin and 0.02% EDTA |
| Doubling Time | ca. 28 hrs | Cell Number in Vial (cells/1ml) | 5.8 x 10^5 |
| Viability at cell freezing (%) | 90.4 | Antibiotics Used | free |
| Passage Number | P5 at cellbank | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, G6PD, MD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |