JCRB0722 L cl 1D

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0722 Cell Name   L cl 1D
Profile   fibroblast, thymidine kinase-deficient, (C3H mouse) Other Name   
Animal   mouse Strain   C3H
Genus   Mus Species   musculus
Sex   M Age   
Identity    Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   TK~
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   skin fibroblast, TK~,(M)
Classify   infinitive Established by   Kit,S. et al.
Registered by   Umeda, M. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   5x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_F739
Reference
Pubmed id:5230304Regulation of pigment synthesis in mammalian cells, as studied by somatic hybridization.
Davidson RL,Ephrussi B,Yamamoto K
Proc Natl Acad Sci U S A. 1966 Nov;56(5):1437-40
Pubmed id:14056989DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
KIT S,DUBBS DR,PIEKARSKI LJ,HSU TC
Exp Cell Res. 1963 Aug;31():297-312

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0722 Cell Name   L cl 1D
LOT No.   032498 Lot Specification   distribution
Medium   Eagle's minimal essential medium with fetal bovine serum (Summit, lot#8.1E+41). Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/sq.cm. Methods for Passages   Cells treated with 0.05% trypsin & 0.02% EDTA when subculture. Medium change every 2-3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   93.3 Antibiotics Used   free
Passage Number   P7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, LDH examined. mouse.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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