JCRB0710 EJ-1

Cell information

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JCRB0710 EJ-1: problem of origin

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0710 Cell Name   EJ-1
Profile   The cell line EJ-1 was identified to be the same cell line as the T24. Other Name   EJ-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   bladder
Case history   bladder carcinoma Metastasis   
Tissue Metastasized    Genetics   STR-PCR data indicated that the EJ-1 is the same cell line as the T24.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Ha-ras mutation reported.
Classify   tumor Established by   Daly,J.
Registered by   Umeda,M. Regulation for Distribution   
Comment   Decontaminated by BM cycline. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   2-3x10^4 cells/ml Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2893
Reference
Pubmed id:6823318Identity of some human bladder cancer cell lines.
O'Toole CM,Povey S,Hepburn P,Franks LM
Nature. 1983 Feb 3;301(5899):429-30
Pubmed id:864752Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.
Marshall CJ,Franks LM,Carbonell AW
J Natl Cancer Inst. 1977 Jun;58(6):1743-51

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0710 Cell Name   EJ-1
LOT No.   041597 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (JRH Biosciences 1B1062). Temperature   37 C
Cell Density at Seeding   1.4x10^4 cells/sq.cm. Methods for Passages   Cells are treated with 0.26% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:12
D7S820:10,11
D16S539:9
VWA:17
TH01:6
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0710 Cell Name   
LOT No.   090095 Lot Specification   distribution
Medium   MEM + 10%FCS Temperature   37
Cell Density at Seeding    Methods for Passages   trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0710 Cell Name   EJ-1
LOT No.   04222024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   5.5 - 7.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:12
D7S820:10,11
D16S539:9
VWA:17
TH01:6
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   
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