JCRB0709 L5178Y
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0709 | Cell Name | L5178Y |
|---|---|---|---|
| Profile | lymphoma, methylcholanthrene induced, (DBA/2 mouse) | Other Name | |
| Animal | mouse | Strain | DBA/2 |
| Genus | Mus | Species | musculus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | lymphoma induced by methylcholanthrene treatment | Metastasis | |
| Tissue Metastasized | Genetics | leukemia | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | culturable derivative of L5178+ |
| Classify | transformed | Established by | Fischer,G.A. |
| Registered by | Umeda,M. | Regulation for Distribution | No regulation was applied to this strain to estab] |
| Comment | Year | 1985 | |
| Medium | Fisher's medium with 10% horse serum. | Methods for Passages | Suspend cells by pipetting. |
| Cell Number on Passage | 2-3 x10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | lymph node |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:3876312 | Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Beer JZ,Mencl J,Horng MF,Gregg EC,Evans HH Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Oct;48(4):609-19 |
| Pubmed id:6883332 | Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Beer JZ,Budzicka E,Niepokojczycka E,Rosiek O,Szumiel I,Walicka M Cancer Res. 1983 Oct;43(10):4736-42 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0709 | Cell Name | |
|---|---|---|---|
| LOT No. | 07032005 | Lot Specification | distribution |
| Medium | Fischer's medium with 10% horse serum | Temperature | 37 C |
| Cell Density at Seeding | 1 - 2 x 10^4 cells/ml | Methods for Passages | dilution |
| Doubling Time | 10 hrs | Cell Number in Vial (cells/1ml) | 5.7 x 10^6 |
| Viability at cell freezing (%) | 92.2 | Antibiotics Used | free |
| Passage Number | Unknown (4 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, AST, LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% horse serum - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |