JCRB0701 FM3A

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0701 Cell Name   FM3A
Profile   mammary carcinoma, spontaneously obtained, (C3H/He mouse) Other Name   FM3A
Animal   mouse Strain   C3H/He
Genus   Mus Species   musculus
Sex   F Age   
Identity    Tissue for Primary Cancer   mammary
Case history    Metastasis   
Tissue Metastasized    Genetics   mammary carcinoma, spontaneously obtained
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   mammary carcinoma, spontaneously obtained
Classify    Established by   Saito,H. & Nakano,N.
Registered by   Umeda,M. Regulation for Distribution   
Comment   High growth rate. PE. near 100 %. Easy to obtain mutant cell line. Year   1985
Medium   ES medium({3051}) with 2% fetal calf serum or E.MEM with 10% fetal calf serum. Methods for Passages   Prepare cell suspension by pipetting and dilute.
Cell Number on Passage   1-2 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   mammary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3869
Reference
Pubmed id:7105014Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate.
Koyama H,Kodama H
Cancer Res. 1982 Oct;42(10):4210-4
Pubmed id:5528803Initiation of a differentiated function (hyaluronic acid synthesis) by hybrid formation in culture.
Koyama H,Ono T
Biochim Biophys Acta. 1970 Oct 15;217(2):477-87
Pubmed id:5956504Long-term culture of the ascites form of a mouse mammary tumor in serum-free media.
Yamane I,Nakano N
Tohoku J Exp Med. 1966 Mar 25;88(3):203-14
Pubmed id:5920633Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium.
Nakano N
Tohoku J Exp Med. 1966 Jan 25;88(1):69-84

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0701 Cell Name   FM3A
LOT No.   043097 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF001). Temperature   37 C
Cell Density at Seeding   2.1x10^4 cells/ml Methods for Passages   Simple dilution.
Doubling Time   10.6 hrs Cell Number in Vial (cells/1ml)   4.6x10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   under examination
Chromosome Mode   NT Chromosome Information   under examination
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0701 Cell Name   FM3A
LOT No.   053095 Lot Specification   FDSC/distribution
Medium   ES medium with 2% fetal bovine serum (JRH12103-78P). (ES medium=Nissui,031406) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   Simple suspension by pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.78x10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   Km
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0701 Cell Name   FM3A
LOT No.   092085 Lot Specification   distribution
Medium   ES+2%FCS or MEM + 10%FCS Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   Prepare cell suspension by pipetting and dilute.
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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