JCRB0628 MRK-nu-1

Cell information

Important Notice(s)

JCRB0628 MRK-nu-1: culture medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0628 Cell Name   MRK-nu-1
Profile   Human mammary carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   46
Identity   available Tissue for Primary Cancer   mammary
Case history    Metastasis   
Tissue Metastasized    Genetics   mammary carcinoma, globular
Life Span    Crisis PDL   
Morphology   lymphocyte-like Character   mammary carcinoma
Classify   tumor Established by   Sekiguchi,M.
Registered by   Sekiguchi,M. Regulation for Distribution   
Comment   his is one of the limited No. of cell lines derived from human mammary carcinoma. Estrogen receptor negative. Easy to manipulate because of the susp. culture. T Year   1987
Medium   DM-160 medium(90 %) with 10% fetal calf serum. DM201 may be available from the IFP(http://www.func-p.co.jp/). Methods for Passages   Simple dilution.
Cell Number on Passage   2.0x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   mammary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1428
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908151
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0628 Cell Name   
LOT No.   02072012 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^5 cells/mL Methods for Passages   Dilution (suspension culture)
Doubling Time   approx. 47 hrs. Cell Number in Vial (cells/1ml)   8.1 x 10^5
Viability at cell freezing (%)   81.8 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0628 Cell Name   
LOT No.   07212011 Lot Specification   distribution
Medium   DM-160 medium with 10% fetal bovine serum (GIBCO Cat.# 10099). DM-160 medium can be substituted by DMEM/F12 mixture. Temperature   37 C
Cell Density at Seeding   1.5 - 1.7 x 10^5 cells/mL Methods for Passages   Simple dilution. This cell line is not adherent.
Doubling Time   slow Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   63.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DM-160 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0628 Cell Name   MRK-nu-1
LOT No.   031696 Lot Specification   distribution
Medium   DM-160 medium (Kyokutoh, Powdered) with 10% fetal bovine serum (JRS C019407). Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   74.2 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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