JCRB0627 KYM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0627 Cell Name   KYM-1
Profile   Human rhabdomyosarcoma cell line derived from neck tumor. Other Name   KYM-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   9-month-old
Identity   available Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   neck tumor, rhabdomyosarcoma
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   neck tumor, rhabdomyosarcoma
Classify   tumor Established by   Sekiguchi,M.
Registered by   Sekiguchi,M. Regulation for Distribution   
Comment   Plasminogen activator, hyaluronic acid and alkaline phosphatase were highly produced. The KYM-1 is unique by STR-PCR among 219 cell lines on 04/22/2001. Year   1986
Medium   DM160 medium with 10% fetal calf serum. Methods for Passages   dilution (because of suspension culture)
Cell Number on Passage   not defined Race   Japanese
CO2 Conc.   5 % Tissue Sampling   neck
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3007
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240166
Reference
Pubmed id:1960402A cytotoxicity assay for tumour necrosis factor using a human rhabdomyosarcoma cell line.
Meager A
J Immunol Methods. 1991 Nov 5;144(1):141-3
Pubmed id:3830265Characterization of a human rhabdomyosarcoma cell strain in tissue culture.
Sekiguchi M,Shiroko Y,Suzuki T,Imada M,Miyahara M,Fujii G
Biomed Pharmacother. 1985;39(7):372-80
Pubmed id:360802Postoperative astigmatism and the operating microscope.
Robertson IF
Adv Ophthalmol. 1978;37():117-21

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0627 Cell Name   
LOT No.   02142012 Lot Specification   distribution
Medium   A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium supplemented with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.4 - 2.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 26 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   63.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0627 Cell Name   
LOT No.   030499 Lot Specification   distribution
Medium   DM-160 medium with 10% fetal calf serum DM-160 may be substituted by DMEM:F12=1:1 Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   Weakly adhesive. Suspend the cells with pipetting and dilute to medium.
Doubling Time   D.T.=ca 41 hrs Cell Number in Vial (cells/1ml)   4.0 x 10^6
Viability at cell freezing (%)   80.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0627 Cell Name   
LOT No.   950206 Lot Specification   distribution
Medium   DM160 + FCS 10% Temperature   37
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   kanamycin 50 ug/ml
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0627 Cell Name   KYM-1
LOT No.   12152021 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F-12 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   0.6 - 1.9 x 10^5 cells/mL Methods for Passages   Weakly adhesive. Cells were harvested by pipetting or by treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 18 hrs. Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
Cell No.   JCRB0627 Cell Name   KYM-1
LOT No.   11022023 Lot Specification   distribution
Medium   DMEM/ F12(1/1) medium (SIGMA, #D8062) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   7.12-7.60x10^6 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.76x10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,14
D13S317:8
D7S820:11
D16S539:9,10
VWA:17
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   75 Additional information   
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