-Detailed Information [JCRB0623]-

JCRB0623 HSC-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0623	Cell Name	HSC-3
Profile	Human oral squamous carcinoma cell line with high metastatic potential.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	64
Identity	available	Tissue for Primary Cancer	oral
Case history		Metastasis
Tissue Metastasized		Genetics	squamous carcinoma, tongue, age 64, (M)
Life Span		Crisis PDL
Morphology		Character	squamous carcinoma, tongue, age 64, (M)
Classify	tumor	Established by	Momose,F.
Registered by	Momose,F.	Regulation for Distribution
Comment		Year
Medium	Eagle's minimal essential medium with 10% calf serum.	Methods for Passages	Cells harvested after 0.25%trypsin and 0.02%EDTA treatment.Subculture with 1/8 dilution once a week.
Cell Number on Passage	1 x 10^5 cells/10cm dish	Race
CO2 Conc.	5 %	Tissue Sampling	tongue
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1288
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	907061

Reference
Pubmed id:9813718	Isolation of a highly metastatic cell line to lymph node in human oral squamous cell carcinoma by orthotopic implantation in nude mice. Matsui T,Ota T,Ueda Y,Tanino M,Odashima S Oral Oncol. 1998 Jul;34(4):253-6
Pubmed id:2228902	Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium. Rikimaru K,Toda H,Tachikawa N,Kamata N,Enomoto S In Vitro Cell Dev Biol. 1990 Sep;26(9):849-56
Pubmed id:2585303	Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas. Momose F,Araida T,Negishi A,Ichijo H,Shioda S,Sasaki S J Oral Pathol Med. 1989 Aug;18(7):391-5

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

07242009
09032003
091985
121498
02022015
07112016
06112018
10242024

Cell No.	JCRB0623	Cell Name	HSC-3
LOT No.	07242009	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% newborn calf serum (GIBCO Cat. # 16010) // Ampoule mislabeled as "lot# 07242008"	Temperature	37 C
Cell Density at Seeding	1 - 2 x 10^5 cells/ml	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 44 hrs	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	96.3	Antibiotics Used	free
Passage Number	Unknown (12 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11,13 D13S317:12 D7S820:13 D16S539:9 VWA:14,17 TH01:6,9.3 AM:X,Y TPOX:8 CSF1PO:11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% CS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0623	Cell Name
LOT No.	09032003	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S)	Temperature	37 C
Cell Density at Seeding	6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	97	Antibiotics Used	free
Passage Number	> P11	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0623	Cell Name	HSC-3
LOT No.	091985	Lot Specification	distribution
Medium	MEM + 10%CS	Temperature	37
Cell Density at Seeding	1 X 10^5	Methods for Passages	trypsin-EDTA, 1:8 dilution once a week
Doubling Time	NT	Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)	NT	Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0623	Cell Name
LOT No.	121498	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% calf serum	Temperature	37 C
Cell Density at Seeding		Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T.=ca. 60 hrs	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	93.0	Antibiotics Used	free
Passage Number	P10*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0623	Cell Name	HSC-3
LOT No.	02022015	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% newborn calf serum (NCS; GIBCO Cat. # 16010)	Temperature	37 C
Cell Density at Seeding	6.1 - 7.3 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 52 hrs	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% newborn calf serum - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

Cell No.	JCRB0623	Cell Name	HSC-3
LOT No.	07112016	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% newborn calf serum (GIBCO Cat. # 16010).	Temperature	37 C
Cell Density at Seeding	1.1 - 1.5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	42 - 65 hrs	Cell Number in Vial (cells/1ml)	1.3 x 10^6
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	Unknown (11 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% newborn calf serum - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	98	Additional information

Cell No.	JCRB0623	Cell Name	HSC-3
LOT No.	06112018	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% newborn calf serum (NCS; Biowest Cat. # S0750)	Temperature	37 C
Cell Density at Seeding	1.6 - 1.8 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 37 hrs.	Cell Number in Vial (cells/1ml)	2.3 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	Unknown (11 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% newborn calf serum - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

Cell No.	JCRB0623	Cell Name	HSC-3
LOT No.	10242024	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% newborn calf serum (NCS; Biowest Cat. # S0750, Lot S14368S0750)	Temperature	37 C
Cell Density at Seeding	3 - 5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 60 hrs.	Cell Number in Vial (cells/1ml)	4.2 x 10^6
Viability at cell freezing (%)	97.2	Antibiotics Used	free
Passage Number	Unknown (11 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11,13 D13S317:12 D7S820:13 D16S539:9 VWA:14,17 TH01:6,9.3 AM:X,Y TPOX:8 CSF1PO:11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% NCS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	98.3	Additional information