JCRB0612 GOTO

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0612 Cell Name   GOTO
Profile   neuroblastoma, adrenal gland Other Name   GOTO
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   neural
Case history    Metastasis   
Tissue Metastasized    Genetics   neuroblastoma
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   neuroblastoma
Classify    Established by   Sekiguchi,M.
Registered by   Sekiguchi,M. Regulation for Distribution   
Comment   Mycoplasma contaminated when submitted to the bank. Year   1985
Medium   RPMI1640 mixed with Eagle's minimal essential medium(1;1) with 10% fetal calf serum Methods for Passages   cells are harvested by rubber policeman
Cell Number on Passage   10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   adrenal gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1234
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 906875
Reference
Pubmed id:470188Establishment and characterization of a human neuroblastoma cell line in tissue culture.
Sekiguchi M,Oota T,Sakakibara K,Inui N,Fujii G
Jpn J Exp Med. 1979 Feb;49(1):67-83

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0612 Cell Name   GOTO
LOT No.   112685 Lot Specification   distribution
Medium   45% RPMI1640 + 45% MEM + 10% FCS Temperature   37
Cell Density at Seeding   10^4 cells/ml Methods for Passages   rubber police man
Doubling Time   48hr Cell Number in Vial (cells/1ml)   1x10^6/ml
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number   P526 PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0612 Cell Name   
LOT No.   052199 Lot Specification   distribution
Medium   RPMI1640 + Eagle's minimal essential medium (1:1) with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1.2 x 10^5 cells/ml Methods for Passages   pipetting-dilution
Doubling Time   D.T.= ca 48 hrs Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0612 Cell Name   GOTO
LOT No.   07132015 Lot Specification   distribution
Medium   RPMI1640 medium + Eagle's minimum essential medium (1:1) with 10% fetal bovine serum (FBS: GIBCO Cat.# 10091) Temperature   37 C
Cell Density at Seeding   0.8 - 1.1 x 10^5 cells/mL Methods for Passages   pipetting and dilution.
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:15
D7S820:8,11
D16S539:11,13
VWA:17,18,19
TH01:9
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640/EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
Cell No.   JCRB0612 Cell Name   GOTO
LOT No.   04212025 Lot Specification   distribution
Medium   RPMI 1640 medium + Eagle's minimum essential medium (1:1) with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.8 - 1.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. (Cells may be detached by PBS wash only.)
Doubling Time   approx. 52 hrs. Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   91.1 Additional information   
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