-Detailed Information [JCRB0611]-

JCRB0611 KATOIII

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0611	Cell Name	KATOIII
Profile	signet ring cell carcinoma	Other Name	KATOIII
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	stomach
Case history		Metastasis
Tissue Metastasized		Genetics	stomach cancer, signet ring cell carcinoma
Life Span		Crisis PDL
Morphology		Character	stomach cancer, signet ring cell carcinoma
Classify		Established by	Sekiguchi,M.
Registered by	Sekiguchi,M.	Regulation for Distribution
Comment		Year	1985
Medium	RPMI1640 medium 45% with 45% Eagle's minimal essential medium and 10% heat inactivated FBS.	Methods for Passages	Simple dilution.
Cell Number on Passage	approx. 2 x 10^5 cells/mL	Race
CO2 Conc.	5 %	Tissue Sampling	stomach
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0371
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	907276

Reference
Pubmed id:3095279	Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel. Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:209229	Establishment of cultured cell lines derived from a human gastric carcinoma. Sekiguchi M,Sakakibara K,Fujii G Jpn J Exp Med. 1978 Feb;48(1):61-8

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

05232008
06302003
012296
062397
12152014
04092025

Cell No.	JCRB0611	Cell Name
LOT No.	05232008	Lot Specification	distribution
Medium	1:1 mixture of RPMI 1640 and Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	approx. 2 x 10^5 cells/ml	Methods for Passages	Dilution. Cultivate as suspension culture. (Some cells are adherant. If needed, adherant cells are harvested by vigorous pipetting or 0.05% trypsin)
Doubling Time	32 hrs	Cell Number in Vial (cells/1ml)	4.2 x 10^6
Viability at cell freezing (%)	90.9	Antibiotics Used	free
Passage Number	P715	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0611	Cell Name
LOT No.	06302003	Lot Specification	distribution
Medium	1:1 mixture of Eagle's MEM and RPMI1640 with heat-inactivated fetal bovine serum	Temperature	37 C
Cell Density at Seeding	1.5 x 10^5 cells/ml	Methods for Passages	Dilution. Cultivate as suspension culture. Some cells are adherent but these are hardly to detach by pipetting, and not used for subcultivation.
Doubling Time	D.T. = 1.5 days	Cell Number in Vial (cells/1ml)	1.3 x 10^6
Viability at cell freezing (%)	95.2	Antibiotics Used	free
Passage Number	P713	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST and LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - basal medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0611	Cell Name	KATOIII
LOT No.	012296	Lot Specification	distribution
Medium	RPMI1640 : Eagle's minimal essential medium = 1 : 1, mixed medium with 10% heat inactivated FBS.	Temperature	37 C
Cell Density at Seeding	2.0x10^5 cells/ml	Methods for Passages	Simple dilution.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.4x10^6
Viability at cell freezing (%)	94.0	Antibiotics Used	Free
Passage Number	P712	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NP, LDH, G6PD (human confirmed)
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0611	Cell Name
LOT No.	062397	Lot Specification	distribution
Medium	1:1 mixture of RPMI1640 medium and Eagle's MEM with 10% heat-inactivated fetal bovine serum	Temperature	37 C
Cell Density at Seeding	2 x 10^5	Methods for Passages	dilution
Doubling Time		Cell Number in Vial (cells/1ml)	3.0 x 10^6
Viability at cell freezing (%)	91.0	Antibiotics Used	free
Passage Number	P713	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0611	Cell Name	KATOIII
LOT No.	12152014	Lot Specification	distribution
Medium	1:1 mixture of RPMI1640 medium and Eagle's minimum essential medium and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	1.3 - 4.4 x 10^5 cells/mL	Methods for Passages	Dilution. Cultivate as suspension culture. (Some cells are adherant. If needed, adherant cells are harvested by vigorous pipetting or 0.05% trypsin)
Doubling Time		Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	84.2	Antibiotics Used	free
Passage Number	P715	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	84.2	Additional information

Images

Cell No.	JCRB0611	Cell Name	KATOIII
LOT No.	04092025	Lot Specification	distribution
Medium	1:1 mixture of RPMI1640 medium and Eagle's minimum essential medium and 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K).	Temperature	37 C
Cell Density at Seeding	2.0 - 5.6 x 10^5 cells/mL	Methods for Passages	Dilution. Cultivate as suspension culture. (Some cells are adherant. If needed, adherant cells are harvested by vigorous pipetting or trypsin treatment.)
Doubling Time	approx. 68 hrs.	Cell Number in Vial (cells/1ml)	3.2 x 10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	P713	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,11 D13S317:8,12 D7S820:8,12 D16S539:10,12 VWA:14,16 TH01:7,9 AM:X TPOX:11 CSF1PO:7,11
Adhesion	Y/N	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	95	Additional information