JCRB0607 MK.P3(F)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0607 | Cell Name | MK.P3(F) |
|---|---|---|---|
| Profile | other name is JTC-12.P3(F) | Other Name | JTC-12.P3(F) |
| Animal | monkey, Cynomolgus | Strain | Cynomologus |
| Genus | Macaca | Species | fascicularis |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | kidney | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Adapted to serum free DM-160 but later cells were maintained by medium containing serum. This is different from the serum free JTC-12.P3(MK.P3). |
| Classify | Established by | Katsuta,H. | |
| Registered by | Kuroki,T. | Regulation for Distribution | |
| Comment | Year | ||
| Medium | DM160 medium. No serum required. | Methods for Passages | Harvest cells with rubber policeman. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:208643 | Hormone-specific responses and biosynthesis of sulfolipids in cell lines derived from mammalian kidney. Ishizuka I,Tadano K,Nagata N,Niimura Y,Nagai Y Biochim Biophys Acta. 1978 Jul 17;541(4):467-82 |
| Pubmed id:351092 | [Topics on sulfated glycolipids (author's transl)]. Ishizuka I Seikagaku. 1978 Mar 25;50(3):145-59 |
| Pubmed id:13984589 | Establishment of a cell strain, JTC-12, from cynomolgus monkey kidney tissue. TAKAOKA T,KATSUTA H,ENDO M,SATO K,OKUMURA H Jpn J Exp Med. 1962 Aug;32():351-68 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0607 | Cell Name | MK.P3(F) |
|---|---|---|---|
| LOT No. | 02072008 | Lot Specification | distribution |
| Medium | DM-160 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). | Temperature | 37 C |
| Cell Density at Seeding | 3 - 6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA Label of ampoule is JTC-12.P3(F). |
| Doubling Time | 18 hrs | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as cynomolgus monkey by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DM-160 medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information | Ampoule label is JTC-12.P3(F) (Old name of this cell line) |
| Cell No. | JCRB0607 | Cell Name | |
|---|---|---|---|
| LOT No. | 890210 | Lot Specification | distribution |
| Medium | DM160 with 10% FCS | Temperature | 37 |
| Cell Density at Seeding | Methods for Passages | rubber policeman | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | 60 ug/ml kanamycin | |
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |