JCRB0506 TIG-3-20

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

TIG-3細胞の性別について

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0506 Cell Name   TIG-3-20
Profile   Normal fibroblast cell line derived from Japanese fetal lung. Other Name   TIG-3
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity    Tissue for Primary Cancer   lung
Case history   normal Metastasis   
Tissue Metastasized    Genetics   fetal lung, (M)
Life Span   finite Crisis PDL   PDL=84
Morphology   fibroblast-like Character   fetal lung, (M)
Classify   normal Established by   Tky.Inst.Geront.
Registered by   Matsuo,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   Subculture every 7 days. Split ratio, 1/4. 0.25 % trypsin, 10-15 min.
Cell Number on Passage   6-8x10^4 cells/cm^2 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3205
Reference
Pubmed id:7053395Ploidy of human embryonic fibroblasts during in vitro aging.
Matsuo M,Kaji K,Utakoji T,Hosoda K
J Gerontol. 1982 Jan;37(1):33-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0506 Cell Name   
LOT No.   06272008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375). This lot was forzen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   19
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0506 Cell Name   
LOT No.   09122003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. This lot was frozen with DMSO. Temperature   37 C
Cell Density at Seeding   split ratio 1:4 - 1:6. Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 1.5 days Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   87.4 Antibiotics Used   free
Passage Number   P3* PDL   27
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0506 Cell Name   TIG-3-20
LOT No.   R033 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   split ratio = 1/4 Methods for Passages   Refer attached infomation.
Doubling Time   NT Cell Number in Vial (cells/1ml)   tow dishes
Viability at cell freezing (%)   NT Antibiotics Used   100 ug/ml streptomysin, 100 units/ml penicillin
Passage Number    PDL   PDL21
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   46,XY Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with glycerol (concentration not described). CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0506 Cell Name   
LOT No.   R032 Lot Specification   distribution
Medium   MEM + 10% FCS (this ampule was freezed using glycerol) Temperature   37
Cell Density at Seeding   6-8x10^4 Methods for Passages   Subculture every 7 days. Split ratio, 1:4. 0.25% trypsin, 10-15 min.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   24PDL
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0506 Cell Name   
LOT No.   R034 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. This lot of ampoule was frozen using glycerol. Temperature   37 C
Cell Density at Seeding   split ratio 1:4 Methods for Passages   0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   5.7 x 10^6
Viability at cell freezing (%)   90.6 Antibiotics Used   100 ug/ml streptomycin and 100 units/ml penicillin
Passage Number    PDL   22
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium containing 10% glycerol. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0506 Cell Name   TIG-3-20
LOT No.   11272014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   3.7 - 4.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = approx. 52 hrs Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   21.6
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B) MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   84.0 Additional information   
Cell No.   JCRB0506 Cell Name   TIG-3-20
LOT No.   07262017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2.4 - 8.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   85.2 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   19.2
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   85.2 Additional information   
Cell No.   JCRB0506 Cell Name   TIG-3-20
LOT No.   02192019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   19.6
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,13
D13S317:9,12
D7S820:10,12
D16S539:10
VWA:16,17
TH01:7,9
AM:X,Y
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   JCRB0506 Cell Name   TIG-3-20
LOT No.   02242023 Lot Specification   distribution
Medium   Eagle's minimum essential medium (GIBCO:　catalogNo.11095-080) ​ with 10% fetal bovine serum (SIGMA, Cat#172012, Lot#12J396, not HI)　 Temperature   37 C
Cell Density at Seeding   1.9-3.6x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025% trypsin-EDTA.　（Split ratio:1/4）
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.9x10^6
Viability at cell freezing (%)   100.00 Antibiotics Used   free
Passage Number   ｐ7* PDL   20.1
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:9,12
D7S820:10,12
D16S539:10
VWA:16,17
TH01:7,9
AM:X,Y
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   97.8 Additional information   
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