JCRB0427 HuO9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0427 Cell Name   HuO9
Profile   A cell line established from a human osteosarcoma transplanted into athymic nude mice after fifth passages. The cell line expressed 1,25(OH)2D3-responsive alikaline phosphatase and produced tumors in the dorsum of nude mice. Other Name   HuO9
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   13
Identity   available Tissue for Primary Cancer   sarcoma
Case history   osteosarcoma Metastasis   
Tissue Metastasized    Genetics   Liver/bone/kidney-type alkaline phosphatase.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Secretes osteocalcin & responds to vitamin D3.
Classify   tumor Established by   Kawai,A.et al.
Registered by   Namba,M. Regulation for Distribution   No commercial purpose.
Comment    Year   
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells treated with 0.2 % trypsin and 0.02% EDTA.
Cell Number on Passage   1-4 x 10^5 cells/ml Race   Jpanese
CO2 Conc.   5 % Tissue Sampling   bone (right femur)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1298
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907072
Reference
Pubmed id:2170070A newly established human osteosarcoma cell line with osteoblastic properties.
Kawai A
Clin Orthop Relat Res. 1990 Oct;(259):256-67
Pubmed id:2691514Two distinct cell lines derived from a human osteosarcoma.
Kawai A,Ozaki T,Ikeda S,Oda T,Miyazaki M,Sato J,Taketa K,Inoue H
J Cancer Res Clin Oncol. 1989;115(6):531-6

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0427 Cell Name   HuO9
LOT No.   081291 Lot Specification   distribution
Medium   RPMI1640 + 0.2% lactalbmin hydrolysate + 10% FBS Temperature   37 C
Cell Density at Seeding   1-4x10^5 Methods for Passages   0.2 % trypsin treatment
Doubling Time   doubling time=5 Cell Number in Vial (cells/1ml)   >10^6 /ml
Viability at cell freezing (%)    Antibiotics Used   kanamycin (60ug/ml)
Passage Number   >20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   not done
Chromosome Mode    Chromosome Information   The cells widely distribute in a triploid range.
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0427 Cell Name   HuO9
LOT No.   04082014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   3.4 - 7.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   P30 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:11
D7S820:10
D16S539:11
VWA:14
TH01:9
AM:X
TPOX:9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92.3 Additional information   
Cell No.   JCRB0427 Cell Name   HuO9
LOT No.   07272020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.7-2.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(1/3 split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   92.30 Antibiotics Used   free
Passage Number   p31 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:11
D7S820:10
D16S539:11
VWA:14
TH01:9
AM:X
TPOX:9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   50.3 Additional information   We recommend to start culture with 25T bottle or 60mm dish,because viability after thawing is about 50%.
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Cell No.   JCRB0427 Cell Name   HuO9
LOT No.   12092024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37C
Cell Density at Seeding   4.2 - 4.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   48 - 73 hrs. Cell Number in Vial (cells/1ml)   7.7 x 10^5
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:11
D7S820:10
D16S539:11
VWA:14
TH01:9
AM:X
TPOX:9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   80 Additional information   
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