JCRB0418 dRLN-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0418 Cell Name   dRLN-4
Profile   liver cell line, 3'-methyl-4-dimethylaminoazobenzene induced, (Donryu rat) Other Name   
Animal   rat Strain   Donryu
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   liver after treatment by 0.06 % DAB
Life Span    Crisis PDL   
Morphology    Character   liver after treatment by 0.06 % DAB
Classify    Established by   Hoshika,T. & Sato,J.
Registered by   Sato,J. Regulation for Distribution   
Comment    Year   1986
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells treated with 0.2 % trypsin and 0.02% EDTA at subculture.
Cell Number on Passage   More than 1x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6537
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0418 Cell Name   
LOT No.   020299 Lot Specification   distribution
Medium   Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   8.0 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 30hrs Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   85% Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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