JCRB0413 HuO-3N1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0413 Cell Name   HuO-3N1
Profile   osteosarcoma, ilium Other Name   HuO-3N1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   osteosarcoma, age 15, (F)
Life Span    Crisis PDL   
Morphology    Character   osteosarcoma, age 15, (F)
Classify    Established by   Yamane,T. & Sato,J.
Registered by   Sato,J. Regulation for Distribution   
Comment   Osteoblastic cell line. Year   
Medium   RPMI-1640 medium with 10% heat inactivated fetal bovine serum. Methods for Passages   Cells are rinsed by PBS(-) and treated with 0.2% trypsin and 0.02% EDTA at room temperature.
Cell Number on Passage   few cells OK Race   
CO2 Conc.   5 % Tissue Sampling   osteosarcoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1297
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909977
Reference
Pubmed id:3862491Establishment and characterization of cell lines derived from a human osteosarcoma.
Yamane T
Clin Orthop Relat Res. 1985 Oct;(199):261-71

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0413 Cell Name   HuO-3N1
LOT No.   11252025 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.2% trypsin and 0.02% EDTA.
Doubling Time   2.5 - 3 days Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
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