JCRB1185 KMS-21BM

Cell information

Important Notice(s)

JCRB1185 KMS-21BM: On the problem of origin.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1185 Cell Name   KMS-21BM
Profile   human myeloma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   62
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   hyperammonaemia Metastasis   Yes
Tissue Metastasized    Genetics   t(11;14)(q13;q32)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   small round-cell morphology, part is multinucleate cell.
Classify   tumor Established by   Otsuki, T.
Registered by   Otsuki, T. Regulation for Distribution   
Comment    Year   2006
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Subculture twice a week by dilution.
Cell Number on Passage   50000 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   myeloma(IgD lamda)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2991
Reference
Pubmed id:11697507Establishment of CD7+ human myeloma sister cell lines, KMS-21-PE and KMS-21-BM, carrying t(11;14) and t(8;14).
Otsuki T,Wada H,Nakazawa N,Taniwaki M,Kouguchi K,Ohkura M,Uehira K,Isoda K,Yata K,Sakaguchi H,Yawata Y,Ueki A,Yamada O
Leuk Lymphoma. 2001 Aug;42(4):761-74
Pubmed id:11039672Cellular biological differences between human myeloma cell lines KMS-12-PE and KMS-12-BM established from a single patient.
Otsuki T,Hata H,Harada N,Matsuzaki H,Yata K,Wada H,Yawata Y,Ueki A,Yamada O
Int J Hematol. 2000 Aug;72(2):216-22

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1185 Cell Name   KMS-21BM
LOT No.   04122007 Lot Specification   distribution2
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   2.5x10^5 cells/ml Methods for Passages   subculture by dilution(pipetting or scraping)
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.3x10^6
Viability at cell freezing (%)   92.1 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,14
D7S820:8,13
D16S539:9
VWA:18
TH01:7,9
AM:X
TPOX:8
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1185 Cell Name   KMS-21BM
LOT No.   02132007 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone ALA12955) Temperature   37 C
Cell Density at Seeding   4.7x10^5 cells/ml Methods for Passages   subculture by dilution(pipetting or scraping)
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.6x10^6
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,14
D7S820:8,13
D16S539:9
VWA:18
TH01:7,9
AM:X
TPOX:8
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1185 Cell Name   KMS-21BM
LOT No.   05272016 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   2-5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). Subculture twice a week by dilution.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   84.6 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HI-FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   84.6 Additional information   
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