JCRB1181 9-15c

Cell information

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M061101 medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1181 Cell Name   9-15c
Profile   Mouse C3H/He cell line. Other Name   CMG, MSCs
Animal   mouse Strain   C3H/He
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   bone marrow
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2006
Medium   M061101 Methods for Passages   0.25% Typsin - 0.53 mM EDTA
Cell Number on Passage   2 x 10^5 cells / 10 cm dish Race   
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6532
Reference
Pubmed id:21155951Lectin microarray analysis of pluripotent and multipotent stem cells.
Toyoda M,Yamazaki-Inoue M,Itakura Y,Kuno A,Ogawa T,Yamada M,Akutsu H,Takahashi Y,Kanzaki S,Narimatsu H,Hirabayashi J,Umezawa A
Genes Cells. 2011 Jan;16(1):1-11
Pubmed id:12878158In vivo cardiovasculogenesis by direct injection of isolated adult mesenchymal stem cells.
Gojo S,Gojo N,Takeda Y,Mori T,Abe H,Kyo S,Hata J,Umezawa A
Exp Cell Res. 2003 Aug 1;288(1):51-9
Pubmed id:10074487Cardiomyocytes can be generated from marrow stromal cells in vitro.
Makino S,Fukuda K,Miyoshi S,Konishi F,Kodama H,Pan J,Sano M,Takahashi T,Hori S,Abe H,Hata J,Umezawa A,Ogawa S
J Clin Invest. 1999 Mar;103(5):697-705

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1181 Cell Name   9-15c
LOT No.   12122006 Lot Specification   distribution
Medium   M061101(MED-SHIROTORI:Cat#61101) Temperature   37 C
Cell Density at Seeding   1.3x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.53mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   NT
Passage Number   p6 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined.Mouse confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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