JCRB1178 KUM5

Cell information

Important Notice(s)

M061101 medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1178 Cell Name   KUM5
Profile   Osteoblastic cell line of mouse C3H. Other Name   
Animal   mouse Strain   C3H/He
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   bone marrow
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2006
Medium   M061101 Methods for Passages   0.25% Trypsin - 0.53 mM EDTA
Cell Number on Passage   2 x 10^5 cells / 10 cm dish Race   
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   Osteoblasts

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5151
Reference
Pubmed id:15996824Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts.
Matsumoto S,Shibuya I,Kusakari S,Segawa K,Uyama T,Shimada A,Umezawa A
Biochim Biophys Acta. 2005 Aug 30;1725(1):57-63
Pubmed id:1373147Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.
Umezawa A,Maruyama T,Segawa K,Shadduck RK,Waheed A,Hata J
J Cell Physiol. 1992 Apr;151(1):197-205

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1178 Cell Name   KUM5
LOT No.   11142006 Lot Specification   distribution
Medium   M061101(MED-SHIROTORI:Cat#61101) Temperature   37 C
Cell Density at Seeding   2.2x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   
Passage Number   p14 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined.Mouse　confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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