JCRB1175 NCR-G4

Cell information

Important Notice(s)

Cultivation of JCRB1166 NCR-G1, JCRB1167 NCR-G2, JCRB1168 NCR-G3, JCRB1175 NCR-G4

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1175 Cell Name   NCR-G4
Profile   Complex type germ cell tumor of human testis origin. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   testis
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   complex type Character   
Classify   tumor Established by   Hata,J.
Registered by   Umezawa,A. Regulation for Distribution   
Comment    Year   2005
Medium   G031101(HIDA) Methods for Passages   The cell is peeled off by pipetting. (It handles it as much as the human embryonic stem cell. )
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   testis (mixed embryonal carcinoma)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3062
Reference
Pubmed id:2562098[Differentiation of human germ cell tumor cells].
Hata J,Fujita H,Ikeda E,Matsubayashi Y,Kokai Y,Fujimoto J
Hum Cell. 1989 Dec;2(4):382-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1175 Cell Name   NCR-G4
LOT No.   11172006 Lot Specification   distribution
Medium   G031101(within serum and antibiotics:MED-SHIROTORI:Cat#31101) Temperature   37 C
Cell Density at Seeding   2.7x10^2 cells/sq.cm Methods for Passages   The cell is peeled off by pipetting,after PBS(-) washing and treatment with Cell Dissociation Buffer enzyme-free,Hanks-based(Invitrogen Cat.No.13150-016)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   88.5 Antibiotics Used   
Passage Number   p12 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8,13
D7S820:10
D16S539:10,14
VWA:16
TH01:7
AM:X,Y
TPOX:8,9
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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