-Detailed Information [JCRB0405]-

JCRB0405 HLF

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0405	Cell Name	HLF
Profile	hepatoma, non-differentiated	Other Name	HLF
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	liver, gallbladder
Case history		Metastasis
Tissue Metastasized		Genetics	hepatoma, non-differentiated, age 68, male
Life Span		Crisis PDL
Morphology		Character	hepatoma, non-differentiated, age 68, male
Classify		Established by	Doi,I. & Sato,J.
Registered by	Sato,J.	Regulation for Distribution
Comment		Year
Medium	Dulbecco's modified Eagle's medium with with 5% fetal bovine serum.	Methods for Passages	Cells are rinsed with PBS(-) and treat with 0.2% trypsin and 0.02% EDTA at room temperature.
Cell Number on Passage	1.5 x 10^4 cells/sq.cm	Race
CO2 Conc.	5 %	Tissue Sampling	liver
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2947

Reference
Pubmed id:52570	Establishment and some biological characteristics of human hepatoma cell lines. Dor I,Namba M,Sato J Gan. 1975 Aug;66(4):385-92

Images

Movies

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

10102012
05242009
07072005
122895
120198
09102020
09092024

Cell No.	JCRB0405	Cell Name
LOT No.	10102012	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with with 5% fetal bovine serum (GIBCO Cat. # 10099).	Temperature	37 C
Cell Density at Seeding	5.8 - 14 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 42 hrs	Cell Number in Vial (cells/1ml)	8.1 x 10^5
Viability at cell freezing (%)	97.6	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0405	Cell Name	HLF
LOT No.	05242009	Lot Specification	distribution
Medium	DMEM with 5% heat inactivated fetal bovine serum(Hyclone GRD0051)	Temperature	37 C
Cell Density at Seeding	1.4x10^2 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.9x10^6
Viability at cell freezing (%)	99.7	Antibiotics Used	free
Passage Number	p7*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11 D13S317:8 D7S820:11 D16S539:9,11 VWA:16 TH01:6,9 AM:X TPOX:8 CSF1PO:10
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0405	Cell Name
LOT No.	07072005	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	2 - 3 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 42 hrs	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	Unknown (P7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS -DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0405	Cell Name	HLF
LOT No.	122895	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 20% calf serum (ICN Flow.1059023)	Temperature	37 C
Cell Density at Seeding	1.7x10^4 cells/sq.cm.	Methods for Passages	Rised cells with PBS(-) once and cells were treated with 0.25% trypsin and 0.02% EDTA at rm temp.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.70x10^6
Viability at cell freezing (%)	97.6	Antibiotics Used	free
Passage Number	P4*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0405	Cell Name
LOT No.	120198	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FCS lot; BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	2-3 x 10^4 cells/ml	Methods for Passages	0.2% trypsin and 0.02% EDTA
Doubling Time	D.T.=ca. 23 hrs	Cell Number in Vial (cells/1ml)	6.4 x 10^5
Viability at cell freezing (%)	88.0	Antibiotics Used	free
Passage Number	P7*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0405	Cell Name	HLF
LOT No.	09102020	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.2- 1.0 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	100	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	97.6	Additional information

Cell No.	JCRB0405	Cell Name	HLF
LOT No.	09092024	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K).	Temperature	37 C
Cell Density at Seeding	approx. 1.6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 28 hrs.	Cell Number in Vial (cells/1ml)	2.2 x 10^6
Viability at cell freezing (%)	93	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11 D13S317:8 D7S820:11 D16S539:9,11 VWA:16 TH01:6,9 AM:X TPOX:8 CSF1PO:10
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	99	Additional information