JCRB0402 RLN-B2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0402 Cell Name   RLN-B2
Profile   normal liver, (Donryu rat) Other Name   B2
Animal   rat Strain   Donryu
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   normal liver
Classify   normal Established by   Masuji,H. & Sato,J.
Registered by   Sato,J. Regulation for Distribution   
Comment   The cell line keeps diploid range after repetitive cloning. No tumorgenic cell line. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.2% trypsin and 0.02% EDTA.
Cell Number on Passage   More than 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5352
Reference
Pubmed id:4280238Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells.
Masuji H,Nakabayashi H,Sato J
Acta Med Okayama. 1974 Aug;28(4):281-93

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0402 Cell Name   
LOT No.   09132010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   90.3 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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