JCRB1148 KMBC-2

Cell information

Important Notice(s)

The growth property of KMBC-2 / KMBC-2の培養特性について

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1148 Cell Name   KMBC-2
Profile   Urinary bladder cancer cell line Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   bladder
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Doubling time for about 60 hours. A round cell proliferates like stripes. Because vacuoles are recognizable in the cytoplasm, secretion production is suggested.
Classify   tumor Established by   Shuin,T.
Registered by   Shuin,T. Regulation for Distribution   
Comment    Year   2005
Medium   Ham F12 medium with 10% Fetal Bovine Serum Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture every one week at split ratio 1:9.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   urinary bladder
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2977
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1148 Cell Name   KMBC-2
LOT No.   03292006 Lot Specification   distribution
Medium   Ham's F12 medium with 10% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   8.9x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   94.8 Antibiotics Used   Free
Passage Number   P8 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined.Human,not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:8,10
D7S820:12
D16S539:9,11
VWA:18
TH01:8,9
AM:X
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1148 Cell Name   KMBC-2
LOT No.   02082017 Lot Specification   distribution
Medium   Ham' F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   6.2 - 8.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 68 hrs. Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   85.4 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   85.4 Additional information   
Cell No.   JCRB1148 Cell Name   KMBC-2
LOT No.   02102022 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.8 - 4.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   3 - 4 days Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   P11 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - F12 CO2 Conc.   5%
Viability immediately after thawing (%)   89.1 Additional information   
Cell No.   JCRB1148 Cell Name   KMBC-2
LOT No.   11122024 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.0 - 1.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   Slow (3-7 days) Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:8,10
D7S820:12
D16S539:9,11
VWA:18
TH01:8,9
AM:X
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
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