JCRB1119 KUSA-A1

Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1119 Cell Name   KUSA-A1
Profile   Mouse osteoblastic cell line. Other Name   
Animal   mouse Strain   C3H
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   bone marrow
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2005
Medium   Poweredby10 Methods for Passages   **
Cell Number on Passage   ** Race   
CO2 Conc.   5% Tissue Sampling   Bone marrow
Tissue Type   osteoblasts

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4848
Reference
Pubmed id:15996824Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts.
Matsumoto S,Shibuya I,Kusakari S,Segawa K,Uyama T,Shimada A,Umezawa A
Biochim Biophys Acta. 2005 Aug 30;1725(1):57-63
Pubmed id:14999559Custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet.
Tsuchiya K,Mori T,Chen G,Ushida T,Tateishi T,Matsuno T,Sakamoto M,Umezawa A
Cell Tissue Res. 2004 May;316(2):141-53
Pubmed id:12447988Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge.
Ochi K,Chen G,Ushida T,Gojo S,Segawa K,Tai H,Ueno K,Ohkawa H,Mori T,Yamaguchi A,Toyama Y,Hata J,Umezawa A
J Cell Physiol. 2003 Jan;194(1):45-53
Pubmed id:1373147Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.
Umezawa A,Maruyama T,Segawa K,Shadduck RK,Waheed A,Hata J
J Cell Physiol. 1992 Apr;151(1):197-205

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1119 Cell Name   KUSA-A1
LOT No.   08192005 Lot Specification   distribution
Medium   POWEREDBY10 Temperature   37 C
Cell Density at Seeding   2.9x10^3 cells/sq.cm Methods for Passages   Harvested after treatment with 0.25% trypsin (GIBCO) and 1mM EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   Free
Passage Number   P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP examined. Mouse confirmed
Chromosome Mode   76 Chromosome Information   71/2 72/1 73/4 74/9 75/9 76/33 77/1 >78/5 (64)
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1119 Cell Name   KUSA-A1
LOT No.   11092015 Lot Specification   distribution
Medium   Poweredby10 medium (GlycoTechnica, Ltd.) Temperature   37 C
Cell Density at Seeding   1.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Poweredby10 CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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