JCRB0262 T.T

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0262 Cell Name   T.T
Profile   Human squamous cell carcinoma derived from esophageal cancer. Other Name   T.T
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   67
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophageal cancer operated. 30Gy irrad. 50mgBMLed. Metastasis   Yes
Tissue Metastasized   oral cavity Genetics   squamous carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   3p-, 6q- Mode=85-87(chr)
Classify   tumor Established by   Takahashi,K. et al.
Registered by   Takahashi, K. Regulation for Distribution   
Comment   Derived from mandibular metastasis. Year   1989
Medium   Dulbecco's modified Eagle's medium with 45% Ham's F12 medium and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.1% trypsin.
Cell Number on Passage   10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   esophagus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3174
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1299064
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0262 Cell Name   
LOT No.   09262006 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.1% trypsin and 0.02% EDTA
Doubling Time   approx. 1.2 days Cell Number in Vial (cells/1ml)   4.6 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P105 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0262 Cell Name   T.T
LOT No.   071195 Lot Specification   FDSC/distribution
Medium   Dulbecco's modified Eagle's medium with Ham's F12(1:1 mix) mixed medium with 10% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   9.8x10^4 cells/ml Methods for Passages   Treat cells with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.82x10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   P101 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0262 Cell Name   T.T
LOT No.   12212001 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P101 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 10% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0262 Cell Name   T.T
LOT No.   10132015 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.5 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   28 - 38 hrs Cell Number in Vial (cells/1ml)   6.0 x 10^6
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   P105 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   92.6 Additional information   
Cell No.   JCRB0262 Cell Name   T.T
LOT No.   07112025 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   6.3 - 8.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   P104 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   
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