JCRB0261 T.Tn
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0261 | Cell Name | T.Tn |
|---|---|---|---|
| Profile | Human squamous cell carcinoma from oral cavity via mouse transplantation. | Other Name | T.Tn |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 67 |
| Identity | available | Tissue for Primary Cancer | esophagus |
| Case history | esophageal cancer operated.30Gy irrad.50mg BMLed. | Metastasis | Yes |
| Tissue Metastasized | Oral cavity | Genetics | High alkaline phosphatase activity. |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Squamous carcinoma. Cells established via mouse being transplanted. |
| Classify | tumor | Established by | Takahashi,K. |
| Registered by | Sato, K. | Regulation for Distribution | |
| Comment | Cells identified to be derived from a same individual by the Cell ID system. EV(T.T/T.Tn)=0.933. | Year | 1989 |
| Medium | 45% Dulbecco's modified Eagle's medium with 45% Ham's F12 medium with 10% fetal calf serum | Methods for Passages | Cells are treated with 0.02% EDTA and 0.1% trypsin. |
| Cell Number on Passage | 10^5 cells/60mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | esophagus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
|---|
     |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0261 | Cell Name | |
|---|---|---|---|
| LOT No. | 01212003 | Lot Specification | distribution |
| Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | 1-1.5 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA for 5-10 min at 37 C. |
| Doubling Time | 32-35 hrs | Cell Number in Vial (cells/1ml) | 4.5 x 10^6 |
| Viability at cell freezing (%) | 94 | Antibiotics Used | free |
| Passage Number | P113 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD, LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS, DMEM:F12 = 1:1 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0261 | Cell Name | |
|---|---|---|---|
| LOT No. | 101494 | Lot Specification | distribution |
| Medium | DMEM + Ham's F12 (1:1) + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 10^5 cells/6cm-dish | Methods for Passages | 0.02% EDTA and 0.1% trypsin. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.5 x 10^6 | |
| Viability at cell freezing (%) | 93.0 | Antibiotics Used | free |
| Passage Number | P106 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | AST, G6PD, LD, MD, MPI, NP, PEPB |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0261 | Cell Name | T.Tn |
|---|---|---|---|
| LOT No. | 01262015 | Lot Specification | distribution |
| Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 0.6 - 1.5 x 10^5 cells/mL | Methods for Passages | Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 28 hrs. | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | P113 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:8,10 D13S317:10,11 D7S820:11 D16S539:9,11 VWA:17 TH01:9 AM:X,Y TPOX:8,11 CSF1PO:11,13 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 93 | Additional information |