JCRB0259 MSK Cell information
Cell type：general cells
(View Pricing Information )
JCRB No.
JCRB0259
Cell Name
MSK
Profile
osteosarcoma, 32P-induced, (F344 rat)
Other Name
MSK
Animal
rat
Strain
Genus
Rattus
Species
norvegicus
Sex
Age
Identity
Tissue for Primary Cancer
sarcoma
Case history
Metastasis
Tissue Metastasized
Genetics
32P-induced osteosarcoma, F344 rat, female
Life Span
infinite
Crisis PDL
Morphology
Character
32P-induced osteosarcoma, F344 rat, female
Classify
Established by
Takahashi,K.
Registered by
Sato, K.
Regulation for Distribution
Contact to K. Sato at distribution of cells.
Comment
High tumorgenecity and metastasis in a F344 rat.
Year
1989
Medium
Dulbecco's modified Eagle's medium with 10% fetal calf serum
Methods for Passages
Cells are treated with 0.02% EDTA and 0.1% trypsin.
Cell Number on Passage
10^5 cells/60mm dish
Race
CO2 Conc.
5 %
Tissue Sampling
osteosarcoma
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.
JCRB0259
Cell Name
MSK
LOT No.
051695
Lot Specification
FDSC/distribution
Medium
dulbecco's modified Eagle's medium with 10% fetal bovine serum (Bio cell, 4001776)
Temperature
37 C
Cell Density at Seeding
1.6x10^5 cells/ml
Methods for Passages
Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
3.6x10^6
Viability at cell freezing (%)
97.2
Antibiotics Used
free
Passage Number
P186
PDL
NT
Sterility: MYCOPLASMA
NT
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
CO2 Conc.
Viability immediately after thawing (%)
Additional information
