JCRB0258 LC-1 sq
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0258 | Cell Name | LC-1 sq |
|---|---|---|---|
| Profile | squamous cell carcinoma | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 69 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | lung, squamous carcinoma | |
| Life Span | infinite | Crisis PDL | NT |
| Morphology | epithelial-like | Character | lung, squamous carcinoma |
| Classify | Established by | Kataoka,H. | |
| Registered by | Kataoka, H. | Regulation for Distribution | Please send a reprint to Kataoka, H. |
| Comment | 69 year-old, male. Moderately differentiated. | Year | 1989 |
| Medium | 45% RPMI1640 medium with 45% Ham's F12 and 10% fetal calf serum | Methods for Passages | Cells are treated with 0.02% EDTA and 0.1% trypsin. |
| Cell Number on Passage | split ratio=1/4,every 2 wks | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1917142 | Establishment of a new human cancer cell line secreting protease nexin-II/amyloid beta protein precursor derived from squamous-cell carcinoma of lung. Itoh H,Kataoka H,Koita H,Nabeshima K,Inoue T,Kangawa K,Koono M Int J Cancer. 1991 Sep 30;49(3):436-43 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0258 | Cell Name | |
|---|---|---|---|
| LOT No. | 11122004 | Lot Specification | distribution |
| Medium | 1:1 mixture of RPMI1640 and Ham's F12 with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 3 - 10 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | ca. 75 hrs | Cell Number in Vial (cells/1ml) | 1.5 x 10^6 |
| Viability at cell freezing (%) | 95.8 | Antibiotics Used | free |
| Passage Number | P55 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B) MD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0258 | Cell Name | |
|---|---|---|---|
| LOT No. | 091093 | Lot Specification | distribution |
| Medium | RPMI 1640 + Ham's F12 (1:1) + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | Methods for Passages | 0.02% EDTA and 0.1% trypsin. | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0258 | Cell Name | LC-1 sq |
|---|---|---|---|
| LOT No. | 06162014 | Lot Specification | distribution |
| Medium | 1:1 mixture of RPMI1640 medium and Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 7.3 - 7.6 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 9.5 x 10^5 | |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | P53 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12,13 D13S317:12,13 D7S820:12 D16S539:9 VWA:17,18 TH01:7,9 AM:X TPOX:8,12 CSF1PO:12 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 99 | Additional information | Mislabeled as LC-1 sp on vials. |
| Cell No. | JCRB0258 | Cell Name | LC-1 sq |
|---|---|---|---|
| LOT No. | 07272022 | Lot Specification | distribution |
| Medium | 1:1 mixture of RPMI1640 medium and Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) | Temperature | 37 C |
| Cell Density at Seeding | 2.4 - 8.8 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 39 hrs. | Cell Number in Vial (cells/1ml) | 2.0 x 10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | P53 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12,13 D13S317:12,13 D7S820:12 D16S539:9 VWA:17,18 TH01:7,9 AM:X TPOX:8,12 CSF1PO:12 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640/F-12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 99 | Additional information |