JCRB0252 MKN1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0252 Cell Name   MKN1
Profile   adenosquamous carcinoma Other Name   MKN1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   72-year-old
Identity   available Tissue for Primary Cancer   stomach
Case history    Metastasis   
Tissue Metastasized    Genetics   stomach, adenosquamous carcinoma, 72 year-old male
Life Span    Crisis PDL   
Morphology    Character   stomach, adenosquamous carcinoma, 72 year-old male
Classify    Established by   Hojo,H.
Registered by   Motoyama & Watanabe Regulation for Distribution   
Comment   Mycoplasma-eliminated cells by MC-210. Year   1989
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25 % trypsin and 0.02 % EDTA.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1415
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908138
Reference
Pubmed id:3095279Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:noneEstablishment of cultured cell lines of human stomach cancer origin and their morphological characteristics.
Hojo H, Oboshi S.
Niigata Igkkai Zasshi, 91: 737-752, 1977.

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0252 Cell Name   
LOT No.   01312012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   4.1 - 6.5 x 10^4 cells/mL Methods for Passages   Cells were treated after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   92.8 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0252 Cell Name   MKN1
LOT No.   07022010 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   3.4-4.8x10^5/100m/m dish Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA and split ratio=1/10
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.91x10^6
Viability at cell freezing (%)   99.7 Antibiotics Used   free
Passage Number   p20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:10,12
D7S820:10
D16S539:11,12
VWA:16
TH01:9
AM:X
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0252 Cell Name   
LOT No.   03302009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   2.3 - 3.4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0252 Cell Name   
LOT No.   09262005 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot: GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 32 hrs Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0252 Cell Name   
LOT No.   072999 Lot Specification   distribution
Medium   RPMI1640 with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca 36 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P119 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0252 Cell Name   MKN1
LOT No.   021496 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (JRS.DO10400) Temperature   37 C
Cell Density at Seeding   1.4x10^5 cells/ml Methods for Passages   Washed cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 10 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P19 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LHD, G6PD, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0252 Cell Name   MKN1
LOT No.   022089 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   87% Antibiotics Used   free
Passage Number   P13* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,G6PD,NP,PepB,AST,MD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0252 Cell Name   
LOT No.   082192 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   5.0 x 10^4
Viability at cell freezing (%)   NT Antibiotics Used   free, Mycoplasma-eliminated previously by MC-210
Passage Number   *P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST, G6PD, LD, MD, MPI, NP, PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0252 Cell Name   MKN1
LOT No.   11192021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   4.0 - 4.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   ~100 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
Cell No.   JCRB0252 Cell Name   MKN1
LOT No.   01202025 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.83-2.5-x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p21 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:10,12
D7S820:10
D16S539:11,12
VWA:16
TH01:9
AM:X
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
Images
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