-Detailed Information [JCRB0251]-

JCRB0251 SCH

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0251	Cell Name	SCH
Profile	choriocarcinoma arising at stomach from male patient	Other Name	SCH
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	stomach
Case history		Metastasis
Tissue Metastasized		Genetics	stomach, choriocarcinoma, 46 year-old male
Life Span		Crisis PDL
Morphology		Character	stomach, choriocarcinoma, 46 year-old male
Classify		Established by	Ohboshi,S.
Registered by		Regulation for Distribution
Comment	Mycoplasmas eliminated by MC-210 in FDSC.	Year	1989
Medium	RPMI1640 medium with 10% fetal calf serum	Methods for Passages	Cells are treated with 0.25 % trypsin and 0.02 % EDTA.
Cell Number on Passage	split ratio=1/10	Race
CO2 Conc.	5 %	Tissue Sampling	stomach
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1687
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	909711

Reference
Pubmed id:3095279	Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel. Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:1170940	A human gastric choriocarcinoma cell line with human chorionic gonadotropin and placental alkaline phosphatase production. Kameya T,Kuramoto H,Suzuki K,Kenjo T,Oshikiri T,Hayashi H,Itakura M Cancer Res. 1975 Aug;35(8):2025-32
Pubmed id:5058640	Use of 1-nitroso-2-naphthol in quantitative determination of medicinal phenolic compounds. Bhansali KG J Pharm Sci. 1972 Jan;61(1):146-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

07022010
102594
04192019
10162023

Cell No.	JCRB0251	Cell Name
LOT No.	07022010	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	1.3 - 1.7 x 10^5 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time	approx. 1.5 days.	Cell Number in Vial (cells/1ml)	2.5 x 10^6
Viability at cell freezing (%)	93.5	Antibiotics Used	free
Passage Number	P21	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0251	Cell Name
LOT No.	102594	Lot Specification	distribution
Medium	RPMI1640 + FCS 10 %	Temperature	37
Cell Density at Seeding		Methods for Passages	0.02 % EDTA and 0.25 % trypsin
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0251	Cell Name	SCH
LOT No.	04192019	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.5 - 1.7 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 28 hrs.	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	79.0	Antibiotics Used	free
Passage Number	P21	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	80.1	Additional information

Cell No.	JCRB0251	Cell Name	SCH
LOT No.	10162023	Lot Specification	distribution
Medium	RPMI1640(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396)	Temperature	37 C
Cell Density at Seeding	0.833-1.12x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.88x10^6
Viability at cell freezing (%)	87.66	Antibiotics Used	free
Passage Number	p21	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11 D13S317:11,12 D7S820:10,11 D16S539:9 VWA:17 TH01:6,7 AM:X,Y TPOX:11 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	87.24	Additional information

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