JCRB0250 NEC8

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0250 Cell Name   NEC8
Profile   testicular germ cell tumor Other Name   NEC8
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   24
Identity   available Tissue for Primary Cancer   testis
Case history    Metastasis   
Tissue Metastasized    Genetics   testicular germ cell tumor, 24 year-old, male
Life Span    Crisis PDL   
Morphology    Character   testicular germ cell tumor, 24 year-old, male
Classify    Established by   Yamamoto,T.
Registered by   Motoyama, T. Regulation for Distribution   
Comment   Mycoplasma-eliminated cells by MC-210. Year   1989
Medium   RPMI1640 medium 10% fetal calf serum Methods for Passages   Cells are treated with 0.05 % trypsin and 0.02 % EDTA.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   testis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1604
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 910942
Reference
Pubmed id:2445169Production of alpha-fetoprotein by human germ cell tumors in vivo and in vitro.
Motoyama T,Watanabe H,Yamamoto T,Sekiguchi M
Acta Pathol Jpn. 1987 Aug;37(8):1263-77
Pubmed id:3618218Human testicular germ cell tumors in vitro and in athymic nude mice.
Motoyama T,Watanabe H,Yamamoto T,Sekiguchi M
Acta Pathol Jpn. 1987 Mar;37(3):431-48

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0250 Cell Name   NEC8
LOT No.   082898 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (Cell Culture Technologies,lot.1604309). Temperature   37 C
Cell Density at Seeding   2.5 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   P16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0250 Cell Name   
LOT No.   062991 Lot Specification   distribution
Medium   RPMI 1640 + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P15 PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0250 Cell Name   NEC8
LOT No.   02182013 Lot Specification   distribution
Medium   RPMI1640 medium 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   split ratio=1/10 Methods for Passages   Cells are treated with 0.05 % trypsin and 0.02 % EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.4 x 10^6 cells / m
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,14
D13S317:12
D7S820:8
D16S539:9,10
VWA:14,15
TH01:6,7
AM:X
TPOX:8,11
CSF1PO:13,15
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS, RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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