JCRB1095 KYSE150

Cell information

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Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1095 Cell Name   KYSE150
Profile   Poorly differentiated squamous carcinoma cell line from human esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   49
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophageal cancer Metastasis   
Tissue Metastasized    Genetics   modal chromosome number = 84XX
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Shimada,Y. et al.
Registered by   Shimada,Y. Regulation for Distribution   
Comment    Year   2003
Medium   Ham's F12 medium with 2% fetal bovine serum. Methods for Passages   
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Tissue Type   poorly differentiated squamous carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1348
Reference
Pubmed id:11520067Gene expression profiling in human esophageal cancers using cDNA microarray.
Kan T,Shimada Y,Sato F,Maeda M,Kawabe A,Kaganoi J,Itami A,Yamasaki S,Imamura M
Biochem Biophys Res Commun. 2001 Aug 31;286(4):792-801
Pubmed id:10995879PTEN/MMAC1 expression in esophageal squamous cell carcinomas.
Ding Y,Shimada Y,Kano M,Itami A,Kawabe A,Maeda M,Li Z,Hong T,Sato F,Kaganoi J,Imamura M
Int J Oncol. 2000 Oct;17(4):695-9
Pubmed id:10362086Induction of human leukocyte antigen (HLA)-A2-restricted and MAGE-3-gene-derived peptide-specific cytolytic T lymphocytes using cultured dendritic cells from an HLA-A2 esophageal cancer patient.
Kanaoka S,Yamasaki S,Okino T,Inoue N,Shimada Y,Kaneko M,Otaka A,Fujii N,Imamura M
J Surg Oncol. 1999 May;71(1):16-21
Pubmed id:8486532Hyperthermic enhancement of cytotoxicity and increased uptake of cis-diamminedichloroplatinum(II) in cultured human esophageal cancer cells.
Miyahara T,Ueda K,Akaboshi M,Shimada Y,Imamura M,Utsumi H
Jpn J Cancer Res. 1993 Mar;84(3):336-40

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1095 Cell Name   KYSE150
LOT No.   12112008 Lot Specification   distribution
Medium   Ham's F12 medium with 2% fetal bovine serum Temperature   37 C
Cell Density at Seeding   6 - 8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free (pretreated with MC-210 and BM cycline)
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8,11
D7S820:10,11
D16S539:9,11
VWA:16,17
TH01:7,9
AM:X
TPOX:8
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20%& FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1095 Cell Name   
LOT No.   08272007 Lot Specification   distribution
Medium   Ham's F12 medium with 2% fetal calf serum lotGIBCI484375 Temperature   37
Cell Density at Seeding   3-4 x10^4cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   85hr Cell Number in Vial (cells/1ml)   2.8 x 10^6 cells
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B) AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1095 Cell Name   KYSE150
LOT No.   12102015 Lot Specification   distribution
Medium   Ham's F12 medium(SIGMA,N6658) with 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.3-1.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA(1/3 split) once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,11
D7S820:10,11
D16S539:9,11
VWA:16,17
TH01:7,9
AM:X
TPOX:8
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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