JCRB0247 RL-34

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0247 Cell Name   RL-34
Profile   normal, collagen-induced growth arrest, (Wistar rat) Other Name   RL-34
Animal   rat Strain   Wistar
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history   normal Metastasis   
Tissue Metastasized    Genetics   liver
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cell growth stopped once adhered to collagen base.
Classify   non-tumor Established by   Okigaki,T.
Registered by   Okigaki, T. Regulation for Distribution   
Comment   Eliminated mycoplasma by MC-210. Year   1989
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.05% trypsin.
Cell Number on Passage   2x10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5583
Reference
Pubmed id:12710953Pax6 autoregulation mediated by direct interaction of Pax6 protein with the head surface ectoderm-specific enhancer of the mouse Pax6 gene.
Aota S,Nakajima N,Sakamoto R,Watanabe S,Ibaraki N,Okazaki K
Dev Biol. 2003 May 1;257(1):1-13
Pubmed id:3552263Adhesion and growth of rat liver epithelial cells on an extracellular matrix with proteins from fibroblast conditioned medium.
Yamada M,Okigaki T,Awai M
Cell Struct Funct. 1987 Feb;12(1):53-62
Pubmed id:144803Lack of involvement of monoamine oxidase inhibition in the lethality of acute poisoning by chlordimeform.
Robinson CP,Smith PW
J Toxicol Environ Health. 1977 Oct;3(3):565-8

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0247 Cell Name   RL-34
LOT No.   070198 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Summit, 8.1E+41). Temperature   37 C
Cell Density at Seeding   1.7x10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.05% trypsin and 0.02% EDTA. Subculture every 4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P17* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP examined (rat).
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0247 Cell Name   RL-34
LOT No.   03022017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.6 - 1.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   83.8 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   83.8 Additional information   
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