JCRB0243 B-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0243 Cell Name   B-1
Profile   Leydig cell tumor, estrogen-dependent, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   testis
Case history   Leydig cell tumor Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   estrogen dependent Leydig cell tumor
Classify    Established by   Sato,B.
Registered by   Sato,B. Regulation for Distribution   
Comment    Year   1988
Medium   Eagle's MEM with 10% fetal calf serum and 10^-8M beta-estradiol and 0.1ug/ml Insulin. Methods for Passages   Cells are treated with 0.01 % trypsin and 0.02 % EDTA.
Cell Number on Passage   5x10^3-10^5 cells/35mm dish Race   
CO2 Conc.   5 % Tissue Sampling   testis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U224
Reference
Pubmed id:3643104Effects of estrogen and vanadate on the proliferation of newly established transformed mouse Leydig cell line in vitro.
Sato B,Miyashita Y,Maeda Y,Noma K,Kishimoto S,Matsumoto K
Endocrinology. 1987 Mar;120(3):1112-20
Pubmed id:6467199Effects of tamoxifen on estrogen-induced enhancement or inhibition of tumor growth in mouse Leydig cell tumor lines.
Sato B,Maeda Y,Nishizawa Y,Noma K,Kishimoto S,Matsumoto K
Cancer Res. 1984 Oct;44(10):4386-91
Pubmed id:464569Large-scale production and concentration of human lymphoid interferon.
Klein F,Ricketts RT,Jones WI,DeArmon IA,Temple MJ,Zoon KC,Bridgen PJ
Antimicrob Agents Chemother. 1979 Mar;15(3):420-7




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0243 Cell Name   
LOT No.   082889 Lot Specification   distribution
Medium   MEM + 10 % FCS + 10^-8M E2 + 0.1 ug/ml Insulin Temperature   37
Cell Density at Seeding    Methods for Passages   0.01 % trypsin + 0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
CLOSE