JCRB1082 KYSE170

Cell information

Important Notice(s)

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1082 Cell Name   KYSE170
Profile   Human squamous cell carcinoma cell line established from esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   53
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophagus cancer, T3N0M0 Stage 2a, squamous cell carcinoma (SCC) Metastasis   No
Tissue Metastasized    Genetics   C-Myc and MDM2 amplification observed. p53 mutation, FHIT.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Xenotransplantation positive.
Classify   tumor Established by   Shimada,Y.
Registered by   Shimada,Y. Regulation for Distribution   
Comment    Year   2003
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Tissue Type   mederately differenciated SCC

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1358
Reference
Pubmed id:11092977Nonrandom chromosomal imbalances in esophageal squamous cell carcinoma cell lines: possible involvement of the ATF3 and CENPF genes in the 1q32 amplicon.
Pimkhaokham A,Shimada Y,Fukuda Y,Kurihara N,Imoto I,Yang ZQ,Imamura M,Nakamura Y,Amagasa T,Inazawa J
Jpn J Cancer Res. 2000 Nov;91(11):1126-33
Pubmed id:10446988Lymph node metastasis is associated with allelic loss on chromosome 13q12-13 in esophageal squamous cell carcinoma.
Harada H,Tanaka H,Shimada Y,Shinoda M,Imamura M,Ishizaki K
Cancer Res. 1999 Aug 1;59(15):3724-9
Pubmed id:9699676Methylation of the 5' CpG island of the FHIT gene is closely associated with transcriptional inactivation in esophageal squamous cell carcinomas.
Tanaka H,Shimada Y,Harada H,Shinoda M,Hatooka S,Imamura M,Ishizaki K
Cancer Res. 1998 Aug 1;58(15):3429-34
Pubmed id:9033652Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:9816044p53 mutation, murine double minute 2 amplification, and human papillomavirus infection are frequently involved but not associated with each other in esophageal squamous cell carcinoma.
Shibagaki I,Tanaka H,Shimada Y,Wagata T,Ikenaga M,Imamura M,Ishizaki K
Clin Cancer Res. 1995 Jul;1(7):769-73
Pubmed id:7913084Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M
Int J Cancer. 1994 Jul 15;58(2):291-7
Pubmed id:8518899Prognostic significance of cell culture in carcinoma of the oesophagus.
Shimada Y,Imamura M
Br J Surg. 1993 May;80(5):605-7
Pubmed id:1728357Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1082 Cell Name   KYSE170
LOT No.   10272004 Lot Specification   distribution
Medium   RPMI1640 and Ham's F12 medium(1/1) with 2% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   4.5x10^4 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   93.2 Antibiotics Used   Free
Passage Number   P691 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with final 10% fetal bovine serum and 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1082 Cell Name   KYSE170
LOT No.   10052015 Lot Specification   distribution
Medium   1:1 mixture of Ham's F12 medium and RPMI1640 medium with 2% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   5.8 - 9.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   19 - 29 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   free
Passage Number   P690 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12,13
D13S317:9
D7S820:11,12
D16S539:9,10
VWA:16,17
TH01:7,9
AM:X
TPOX:11
CSF1PO:10,12,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   96.8 Additional information   
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