JCRB0612.1 GOTO.P3
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0612.1 | Cell Name | GOTO.P3 |
|---|---|---|---|
| Profile | Serum free cell culture of neuroblastoma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 13-month-old |
| Identity | Tissue for Primary Cancer | neural | |
| Case history | neuroblastoma apeared in the left adrenal gland. | Metastasis | |
| Tissue Metastasized | Genetics | neroblastoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | GOTO cells cultured with serum free medium for long period by serial subcultures. |
| Classify | tumor | Established by | Takaoka,T. & Sekiguchi,M. |
| Registered by | Takaoka,T. | Regulation for Distribution | |
| Comment | Year | 2004 | |
| Medium | Serum free DM201 medium. | Methods for Passages | Cells are harvested by rubber policeman gently. |
| Cell Number on Passage | 10^4 cells/sq.cm. | Race | Japanese |
| CO2 Conc. | Tissue Sampling | adrenal grand | |
| Tissue Type | neuroblastoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:470188 | Establishment and characterization of a human neuroblastoma cell line in tissue culture. Sekiguchi M,Oota T,Sakakibara K,Inui N,Fujii G Jpn J Exp Med. 1979 Feb;49(1):67-83 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0612.1 | Cell Name | GOTO.P3 |
|---|---|---|---|
| LOT No. | 08272004 | Lot Specification | distribution |
| Medium | DM201 medium. Serum Free. | Temperature | 37 C |
| Cell Density at Seeding | 1.0-2.5x10^4 cells/sq.cm. | Methods for Passages | Harvest cells with scraper. Subculture every 7-10 days and medium change every 2-4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.2 x 10^6 |
| Viability at cell freezing (%) | 71.5 | Antibiotics Used | MC210 treated on this culture only. (3 weeks). |
| Passage Number | P4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:15 D7S820:8,11 D16S539:13 VWA:17,19 TH01:9 AM:X TPOX:11 CSF1PO:10 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 1 to 1 mixture of the culture medium and the FM-1 medium containing 10% DMSO(final). | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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