-Detailed Information [JCRB0240]-

JCRB0240 MCAS

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0240	Cell Name	MCAS
Profile	mucinous cystadenocarcinoma	Other Name	MCAS
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	ovary
Case history		Metastasis
Tissue Metastasized		Genetics
Life Span		Crisis PDL
Morphology		Character
Classify		Established by	Kidera,Y.
Registered by	Iwasaka,T.	Regulation for Distribution
Comment		Year	1988
Medium	Eagle's minimal essential medium with 20% fetal calf serum	Methods for Passages	0.025 % EDTA-0.02 % trypsin, 3-5 min at 37 C. Split ratio = 1/5
Cell Number on Passage	5.4x10^4 cells/cm^2	Race
CO2 Conc.	5 %	Tissue Sampling	ovary
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3020

Reference
Pubmed id:4056530	[Establishment and characterization of a cell line derived from mucinous cystadenocarcinoma of human ovary]. Kidera Y,Yoshimura T,Ohkuma Y,Iwasaka T,Sugimori H Nihon Sanka Fujinka Gakkai Zasshi. 1985 Sep;37(9):1820-4

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

01202012
03262008
062395
08162016
12082023

Cell No.	JCRB0240	Cell Name	MCAS
LOT No.	01202012	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% fetal bovine serum (FBS, GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	1.5 - 3.6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Pssage twice a week.
Doubling Time	approx. 20 hrs.	Cell Number in Vial (cells/1ml)	1.0 x 10^6
Viability at cell freezing (%)	96.3	Antibiotics Used	free
Passage Number	P22	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	90%	Additional information

Cell No.	JCRB0240	Cell Name
LOT No.	03262008	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% fetal bovine serum (GIBCO Cat.#10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	1 - 2 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA at 37 C for 10 min.
Doubling Time	approx. 28 hrs	Cell Number in Vial (cells/1ml)	6.7 x 10^5
Viability at cell freezing (%)	91.5	Antibiotics Used	free
Passage Number	P23	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0240	Cell Name	MCAS
LOT No.	062395	Lot Specification	distribution
Medium	Eagle's minimal essential medium with fetal bovine serum (20%) (JRH12103-78P)	Temperature	37 C
Cell Density at Seeding	2.1x10^4 cells/ml	Methods for Passages	Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.5 x 10^5
Viability at cell freezing (%)	95.1	Antibiotics Used	free
Passage Number	P20	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0240	Cell Name	MCAS
LOT No.	08162016	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.5 - 1.8 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 39 hrs.	Cell Number in Vial (cells/1ml)	1.8 x 10^6
Viability at cell freezing (%)	87.1	Antibiotics Used	free
Passage Number	Unknown (22 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	87.1	Additional information

Cell No.	JCRB0240	Cell Name	MCAS
LOT No.	12082023	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 20% fetal bovine serum (FBS; NICHIREI Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	approx. 2.7 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 44 hrs.	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	100	Antibiotics Used	free
Passage Number	P22	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11,13 D13S317:8,12 D7S820:8,11 D16S539:12 VWA:14,17 TH01:6,8 AM:X TPOX:8,11 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	100	Additional information

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