JCRB1081 MS-1-L
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1081 | Cell Name | MS-1-L |
|---|---|---|---|
| Profile | Human lung small cell carcinoma from pleural effusion. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 54 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | lung cancer | Metastasis | |
| Tissue Metastasized | Genetics | Chromosome distribution is between 49 and 52. 1p+, 3q-, 6p-, 14p+, 17p+. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | cluster formation observed | Character | Cells are producing PTHrP. |
| Classify | tumor | Established by | Masuda,N. |
| Registered by | Iguchi,H. | Regulation for Distribution | free |
| Comment | Original name of this cell line(MS-1) was changed to the MS-1-L because of the same name existence, CRL2279(mouse). | Year | 2004 |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | Simple dilution |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | pleural effusion |
| Tissue Type | lung small cell carcinoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9183634 | [A PTHrP-producing cell line derived from human small cell lung carcinoma]. Iguchi H Hum Cell. 1996 Mar;9(1):75-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1081 | Cell Name | |
|---|---|---|---|
| LOT No. | 10302008 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) | Temperature | 37 C |
| Cell Density at Seeding | 5 x 10^4 cells/mL | Methods for Passages | dilusion (suspension culture) |
| Doubling Time | 42 hrs | Cell Number in Vial (cells/1ml) | 4.3 x 10^6 |
| Viability at cell freezing (%) | 91 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1081 | Cell Name | MS-1-L |
|---|---|---|---|
| LOT No. | 07162004 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal bovine serum (Intergen RB52305). | Temperature | 37 C |
| Cell Density at Seeding | 1.6 x 10^5 cells/ml | Methods for Passages | dilution. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.6 x 10^6 |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | P7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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