JCRB0238 B8/3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0238 Cell Name   B8/3
Profile   Mouse erythroleukemia cell line. Other Name   MEL, Friend
Animal   mouse Strain   DBA/2
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   erythroleukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify    Established by   Ostertag,W.
Registered by   Obinata,M. Regulation for Distribution   
Comment   Possible model system for de-differentiation. Year   1988
Medium   Eagle's minimal essential medium or ES medium (Nissui) with 10% fetal bovine serum. Methods for Passages   Subculture every 4 days.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6368
Reference
Pubmed id:1057176Induction of endogenous and of spleen focus-forming viruses during dimethylsulfoxide-induced differentiation of mouse erythroleukemia cells transformed by spleen focus-forming virus.
Dube SK,Pragnell IB,Kluge N,Gaedicke G,Steinheider G,Ostertag W
Proc Natl Acad Sci U S A. 1975 May;72(5):1863-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0238 Cell Name   
LOT No.   10062000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; Bioscience 40039) Temperature   37 C
Cell Density at Seeding   2-10 x 10^4 cells/ml Methods for Passages   Dilution
Doubling Time   rapid Cell Number in Vial (cells/1ml)   6.0 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P35* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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